Wait a second...
You are here: UCT Praguestudy.vscht.cz → PhD Double Degree (DD) → Available PhD DD Theses

PhD topics for academic year 2021/2022

Department of Biochemistry and Microbiology

Faculty of Food and Biochemical Technology

RNA metabolism related to the control of gene expression in the oocyte and embryo.

Kubelka Michal, Ing. CSc. ( kub...@iapg.cas.cz)
Šušor Andrej, Ing. Ph.D. ( s...@iapg.cas.cz)
Fully grown oocyte utilizes only transcripts synthesized during earlier development. Mechanisms of mRNA metabolism in the mammalian oocyte are still far from being fully understood. In the first meiotic division, shortly after nuclear envelope breakdown, translational hotspots develop in the chromosomal area and in a region which previously surrounded the nucleus. This hotspot is controlled by the activity of the mTOR/4F axis. We suppose that the localization of specific mRNAs and their in situ translation is essential for the generation of developmentally competent oocyte. Thus, the objective of the current project is to investigate translation regulation and polysome profiling of the oocyte after NEBD. We will test our hypothesis that translation of specific mRNAs is essential for meiotic progression before first polar body extrusion. This represents a first attempt to unveil novel regulators of mTOR/4F axis and will add substantial information concerning translational control of specific transcripts after NEBD in order to understand molecular physiology of mammalian oocyte. The aims of project: High-throughput translation analysis after resumption of meiosis in the mammalian oocyte. Regulation of the mTOR/eIF4E pathway after nuclear envelope breakdown..Translation and genomic stability in the mammalian oocyte.Main methods: Next Generation Sequencing, biochemistry, molecular biology, FISH.
Institute of Animal Physiology and Genetics of the CAS, v. v. i.
Faculty of Food and Biochemical Technology

RNA metabolism related to the control of gene expression in the oocyte and embryo.

Kubelka Michal, Ing. CSc. ( kub...@iapg.cas.cz)
Šušor Andrej, Ing. Ph.D. ( s...@iapg.cas.cz)
Fully grown oocyte utilizes only transcripts synthesized during earlier development. Mechanisms of mRNA metabolism in the mammalian oocyte are still far from being fully understood. In the first meiotic division, shortly after nuclear envelope breakdown, translational hotspots develop in the chromosomal area and in a region which previously surrounded the nucleus. This hotspot is controlled by the activity of the mTOR/4F axis. We suppose that the localization of specific mRNAs and their in situ translation is essential for the generation of developmentally competent oocyte. Thus, the objective of the current project is to investigate translation regulation and polysome profiling of the oocyte after NEBD. We will test our hypothesis that translation of specific mRNAs is essential for meiotic progression before first polar body extrusion. This represents a first attempt to unveil novel regulators of mTOR/4F axis and will add substantial information concerning translational control of specific transcripts after NEBD in order to understand molecular physiology of mammalian oocyte. The aims of project: High-throughput translation analysis after resumption of meiosis in the mammalian oocyte. Regulation of the mTOR/eIF4E pathway after nuclear envelope breakdown..Translation and genomic stability in the mammalian oocyte.Main methods: Next Generation Sequencing, biochemistry, molecular biology, FISH.
Institute of Animal Physiology and Genetics of the CAS, v. v. i.
Updated: 11.12.2019 11:36, Author: Jan Kříž

UCT Prague
Technická 5
166 28 Prague 6 – Dejvice
IČO: 60461373
VAT: CZ60461373

Czech Post certified digital mail code: sp4j9ch

Copyright: UCT Prague 2017
Information provided by the Department of International Relations and the Department of R&D; technical support by the computing centre.
switch to full version