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PhD topics for academic year 2021/2022

Department of Chemical Engineering

Faculty of Chemical Engineering

Advanced formulation approaches for topical delivery

Štěpánek František, prof. Ing. Ph.D. ( Fra...@vscht.cz)
Zbytovská Jarmila, doc. Mgr. Dr.rer.nat. ( zby...@vscht.cz)
Although skin appears to be a macroscopically homogeneous and biologically passive structure, it is exactly the opposite: it is incredibly heterogeneous both chemically and structurally, and it is host to a diversity of active cells such as macrophages and bacteria. Traditional approaches to topical delivery have relied on relatively simple systems such as passive diffusion from water- or oil-based solutions or creams/gels. The aim of this project is to investigate bioactive transport as a mechanism for topical delivery and find a solution to such molecules as therapeutic peptides, which are known to be extremely challenging to formulate and delivery to the body. This project will explore the use of drug delivery systems that are actively phagocytised for targeting macrophages residing in the skin. These drug delivery systems will include naturally sourced polysaccharide shells or lipidic vesicles obtained from single-cell organisms. Their mild immunogenicity, biocompatibility and ability to encapsulate a broad range of molecules will be utilized for the formulation of APIs that have proven to be challenging by traditional means.

Synthesis and Production of Drugs - Drugs and Biomaterials

Faculty of Chemical Engineering

Advanced formulation approaches for topical delivery

Štěpánek František, prof. Ing. Ph.D. ( Fra...@vscht.cz)
Zbytovská Jarmila, doc. Mgr. Dr.rer.nat. ( zby...@vscht.cz)
Although skin appears to be a macroscopically homogeneous and biologically passive structure, it is exactly the opposite: it is incredibly heterogeneous both chemically and structurally, and it is host to a diversity of active cells such as macrophages and bacteria. Traditional approaches to topical delivery have relied on relatively simple systems such as passive diffusion from water- or oil-based solutions or creams/gels. The aim of this project is to investigate bioactive transport as a mechanism for topical delivery and find a solution to such molecules as therapeutic peptides, which are known to be extremely challenging to formulate and delivery to the body. This project will explore the use of drug delivery systems that are actively phagocytised for targeting macrophages residing in the skin. These drug delivery systems will include naturally sourced polysaccharide shells or lipidic vesicles obtained from single-cell organisms. Their mild immunogenicity, biocompatibility and ability to encapsulate a broad range of molecules will be utilized for the formulation of APIs that have proven to be challenging by traditional means.

Faculty of Chemical Engineering

Application of molecular modelling in the screening and characterization of new solid forms of drug substances

Šoóš Miroslav, prof. Ing. Ph.D. ( Mir...@vscht.cz)
Heyda Jan, RNDr. Mgr. Ph.D. ( jan...@vscht.cz)
Experimental screening of new solid forms of drug substances, i.e. polymorphs, salts, co-crystals or solvates, is very labor process requiring of testing various conditions. Once the new solid form is discovered it is analyzed by a combination of several techniques including XRD, NMR, Raman spectroscopy, SEM, DSC, solubility and stability. In this project we plan to utilize molecular simulations to support experimental screening procedure. This will increase our fundamental understanding of involved interactions between drug and excipient molecules. In particular, we plan to use molecular modelling in the calculation of the interaction energies of prepared drug solid form to rank relative thermodynamic stability and melting temperature. When possible, molecular dynamics simulation will be benchmarked to experimentally measured properties of drug solid forms, i.e. XRD data, or to interactions determined by NMR or FTIR. In the last part, we plan to apply molecular modelling in the description of solubility of newly discovered solid forms in the presence of various excipients (i.e. surfactants, polymers, partner molecules) in water media.
Faculty of Chemical Engineering

Application of molecular modelling in the screening and characterization of new solid forms of drug substances

Šoóš Miroslav, prof. Ing. Ph.D. ( Mir...@vscht.cz)
Heyda Jan, RNDr. Mgr. Ph.D. ( jan...@vscht.cz)
Experimental screening of new solid forms of drug substances, i.e. polymorphs, salts, co-crystals or solvates, is very labor process requiring of testing various conditions. Once the new solid form is discovered it is analyzed by a combination of several techniques including XRD, NMR, Raman spectroscopy, SEM, DSC, solubility and stability. In this project we plan to utilize molecular simulations to support experimental screening procedure. This will increase our fundamental understanding of involved interactions between drug and excipient molecules. In particular, we plan to use molecular modelling in the calculation of the interaction energies of prepared drug solid form to rank relative thermodynamic stability and melting temperature. When possible, molecular dynamics simulation will be benchmarked to experimentally measured properties of drug solid forms, i.e. XRD data, or to interactions determined by NMR or FTIR. In the last part, we plan to apply molecular modelling in the description of solubility of newly discovered solid forms in the presence of various excipients (i.e. surfactants, polymers, partner molecules) in water media.
Faculty of Chemical Engineering

Bioreactors Design Parameters - Experimental study of transport characteristics in various apparatuses

Moucha Tomáš, prof. Dr. Ing. ( mou...@vscht.cz)
Zedníková Mária, Ing. Ph.D. ( zed...@vscht.cz)
The efficiency of new biotechnology and pharmaceutical products manufacture also depends on a suitable bioreactor selection. In the design of an optimal bioreactor, they key parameters are the maximum yield of a primary product and, simultaneously, the lifetime of the used microorganisms. The aim of the doctoral study is to compare the design parameters (transport characteristics such as volumetric mass transfer coefficient, gas hold-up and energy dissipation intensity) of three types of the most commonly used bioreactors. The results will be used to characterize the differences and similarities of specific types of bioreactors in terms of gas distribution, mass transfer and mixing depending on the total energy supplied to the system. Transport characteristics will be obtained experimentally for model batches, which will be designed based on physical properties of real broths.
The work is intended as the cooperation of UCT Prague (supervisor's workplace) with ICPF Prague (consultant's workplace) and appropriatley complements the second PhD topic offered by the consultant. Both cooperating workplaces are equipped by necessary facilities i) mechanically stirred reactor, ii) bubble column and iii) air-lift reactor. All bioreactors are adapted to measure transport characteristics by the same methods, therefore the results will be comparable.
Requirements for an applicant: master degree in chemical or mechanical engineering, organic technology, biotechnology etc.; ability for teamwork; systematic and creative approach to scientific problems; interest in experimental work
Development principles: 1. Prostudovat odbornou literaturu na téma hydrodynamika a přenos hmoty v mechanicky míchaných nádobách, probublávaných kolonách a air-lift reaktorech.
2. Osvojit si techniky měření transportních charakteristik (parametry charakterizující promíchávání v bioreaktorech, objemový koeficient přestupu hmoty, zádrž plynu aj.).
3. Provést systematická měření transportních charakteristik v různých modelových vsádkách a ve třech typech bioreaktorů: mechanicky míchaný reaktor, probublávaná kolona a air-lift reaktor.
4. Na základě získaných výsledků porovnat jednotlivé typy reaktorů, charakterizovat společné i rozdílné vlastnosti a vyhodnotit vhodnost jejich použití.
Faculty of Chemical Engineering

Cahn-Hilliard Models for Evolution of Material Morphology

Kosek Juraj, prof. Dr. Ing. ( Jur...@vscht.cz)
Zubov Alexandr, Ing. Ph.D. ( Ale...@vscht.cz)
The development of new materials for applications in catalysis, construction, cars and local energy storage is the actual area of research. This development is mostly empirical. The objective of this project is the classification of knowledge about the evolution of material structure, the formulation of relevant physico-chemical models and their solution with the goal of obtaining general knowledge. Cahn-Hilliard models constitute one of the general approaches for the simulation of morphology evolution and they are applicable especially for the spinodal decomposition.

For practical and more general applicability of these models we have to develop thoroughly validated models extended about: (i) coupling with CFD simulations and behavior of liquid phase(s), (ii) advanced thermodynamics, (iii) considering the molecular architecture of polymers, (iv) models of transport coefficients dependent on local concentration, temperature and viscosity, (v) advanced description of phase interfaces, (vi) generalized Maxwell-Stefan transport, and (vii) generalization for self-consistent description of problems involving nucleation. Advanced Cahn-Hilliard models will be applied for: (i) morphology optimization of nano-/micro-cellular polymers with the goal of improved application properties, (ii) nucleation and coalescence of bubbles in early stages of foam evolution, (iii) evolution of particle morphology in emulsion copolymerizations, and (iv) evolution of salami morphology of high-impact polystyrene during its continuous manufacturing.

Prospective PhD student will not only improve his/her skills in modeling and visualization of spatially 3D problems, but will also penetrate deeply into physico-chemical nature of studied problems. PhD students will continue the development of modeling tools available in the research group. This project shall be sponsored by EU project H2020, grants and by industrial collaborations. PhD student shall spent part of the study at a foreign institution.

Info: phone 220 44 3296, office B-145, e-mailjkk@vscht.cz, webhttp://kosekgroup.cz
Faculty of Chemical Engineering

CFD modelling and experimental characterization of spray drying proces

Šoóš Miroslav, prof. Ing. Ph.D. ( Mir...@vscht.cz)
Low solubility of drugs represent significant drawback in development of new drug products. Possibility to improve this limitation is formulation of drug molecules in amorphous forms, e.g. using hot-melt extrusion, precipitation process or spray drying process. This project will focus on the preparation of amorphous solid forms of drugs using spray drying process. PhD student will be responsible for process modelling using Computational Fluid Dynamic (CFD) and experimental characterization of the process parameters. Developed model will be based on Euler-Lagrangian approach, where gas will be modeled as continuous phase while formed droplets will be tracked as individual entities. Atomization of liquid stream and formation of droplets will be described by combination of VOF-to-DPM approach allowing to model initial formation of droplets followed by their transformation into solid particles. Modelling effort will be combined with the experimental work, where we plan to characterize atomization conditions as well as properties of formed particles using on-line sersors for measurement of their size and shape. Impact of process parameters, such as gas and liquid flow rates, viscosity of liquid phase, and temperature and relative humidity during spray drying will be considered. In the last stage of the project, gained knowledge will be used to scale up of the process from laboratory scale to pilot scale size.
Faculty of Chemical Engineering

CFD simulation of fire suppression In enclosed spaces

Jahoda Milan, doc. Dr. Ing. ( jah...@vscht.cz)
Stable fire extinguishers are standard fire protection. In the regular arrangement of the fire section, the location of the nozzles is given normatively. In the case of a nonstandard geometry arrangement or when protecting equipment with highly flammable substances, it is necessary to solve the design of the nozzles' position and function using advanced fire engineering analysis methods. These methods include computational fluid dynamics, CFD. The dissertation is focused on CFD modelling the effect of stable mist equipment on fire suppression in closed objects. The work aims to find the model's critical parameters and their values for calculating the nozzles' location for optimal fire protection.
Faculty of Chemical Engineering

Continuous preparation of multicomponent drug solid forms

Šoóš Miroslav, prof. Ing. Ph.D. ( Mir...@vscht.cz)
Skořepová Eliška, Ing. Ph.D. ( sko...@seznam.cz)
Screening of new solid forms is typically done in small scale systems including shaken flasks, evaporating systems, ball mills etc., which by design operate in a batch model. Once new solid form is discovered scaling its production is often very complex task. In this project we plan to test capability to utilize the rotary extruder to prepare multicomponent solid forms of drug substances such as salts, co-crystals or co-amorphs. Initially we will use ball mill to prepare new solid forms of selected drug substance. Upon characterization we will upscale of the production process to the application of extrusion, where same form of the drug substance will be prepared in a continuous mode. Detailed investigation of the process parameters will be done to optimize the production process. Both products will be thoroughly characterized including XRD, NMR, Raman spectroscopy, DSC, SEM, particle characterization, measurement of dissolution and stability testing.
Faculty of Chemical Engineering

Continuous preparation of multicomponent drug solid forms

Šoóš Miroslav, prof. Ing. Ph.D. ( Mir...@vscht.cz)
Skořepová Eliška, Ing. Ph.D. ( sko...@seznam.cz)
Screening of new solid forms is typically done in small scale systems including shaken flasks, evaporating systems, ball mills etc., which by design operate in a batch model. Once new solid form is discovered scaling its production is often very complex task. In this project we plan to test capability to utilize the rotary extruder to prepare multicomponent solid forms of drug substances such as salts, co-crystals or co-amorphs. Initially we will use ball mill to prepare new solid forms of selected drug substance. Upon characterization we will upscale of the production process to the application of extrusion, where same form of the drug substance will be prepared in a continuous mode. Detailed investigation of the process parameters will be done to optimize the production process. Both products will be thoroughly characterized including XRD, NMR, Raman spectroscopy, DSC, SEM, particle characterization, measurement of dissolution and stability testing.
Faculty of Chemical Engineering

Controlling drug crystals properties during crystallization and their impact on consequent unit operations

Šoóš Miroslav, prof. Ing. Ph.D. ( Mir...@vscht.cz)
Ridvan Luděk, Ing. Ph.D. ( lud...@zentiva.cz)
Active Pharmaceutical Ingredients (APIs) are commonly small molecules, which are prepared by chemical synthesis followed by crystallization process. Properties of prepared crystals (i.e. physico-chemical but also formulation properties) are strongly dependent on the used drug solid form, their size and crystal morphology. Therefore, the focus of this project is to study impact of crystallization process parameters and post-processing step on the prepared drug crystals with respect to size, morphology and polymorphism. Temperature modulated batch crystallization will be combined with wet-milling process to control the shape as well as flow properties of prepared drug crystals. Crystallization step will be combined with filtration and drying, to evaluate the impact of the crystal size and shape on the efficiency of these unit operations. In parallel, we will also study impact of washing step on the amount of remaining solvent and the polymorphic stability of the final product. While pharmaceutical industry is typically using batch operation, as a part of this project we will investigate the possibility to prepare same drug crystals as studied in batch mode in a continuous process. Process analytical technology capable to measure crystal size, shape and morphology combined will analysis of composition via Raman spectroscopy will be used to ensure constant product quality. On-line measurement will be supported by off-line measurement via SEM, IR spectroscopy, XRD and NMR. Student will be also involved in the scale up of developed process.
Faculty of Chemical Engineering

Controlling drug crystals properties during crystallization and their impact on consequent unit operations

Šoóš Miroslav, prof. Ing. Ph.D. ( Mir...@vscht.cz)
Skořepová Eliška, Ing. Ph.D. ( sko...@seznam.cz)
Active Pharmaceutical Ingredients (APIs) are commonly small molecules, which are prepared by crystallization process. Properties of prepared crystals (i.e. physico-chemical but also formulation properties) are strongly dependent on the used drug solid form, their size and crystal morphology. Therefore, the focus of this project is to study impact of crystallization process parameters and post-processing step on the prepared drug crystals with respect to size, morphology and polymorphism. Temperature modulated batch crystallization will be combined with wet-milling process to control the shape as well as flow properties of prepared drug crystals. Crystallization step will be combined with following steps, i.e. filtration and drying, to evaluate the impact of the crystal size and shape on the efficiency of these unit operations. In parallel, we will also study impact of washing step on the amount of remaining solvent and the polymorphic stability of the final product. While pharmaceutical industry is typically using batch operation, as a part of this project we will investigate the possibility to prepare same drug crystals as studied in batch mode in a continuous process. Process analytical technology capable to measure crystal size, shape and morphology com will analysis of composition via Raman spectroscopy will be used to ensure constant product quality. On-line measurement will be supported by off-line measurement via SEM, IR spectroscopy, XRD and NMR. Student will be also involved in the scale up of developed process.
Faculty of Chemical Engineering

CO2 capture. Industrial process optimization.

Moucha Tomáš, prof. Dr. Ing. ( mou...@vscht.cz)
CO2 capture belongs to frequent industrial needs, both in the cases of low concentration CO2 removal from waste gases and in the cases of main process streams, e.g., in hydrogen production, when high amount/concentration is to be removed. Just the last example represents the process, which the PhD thesis will be focused to. In the premises of Unipetrol company, the CO2 capture unit is presumed to be permanently optimized. In accordance with the needs of the industrial partner, the experimental research goals will involve i) durability / degradability of the solution currently used in the process, ii)absorption efficiencies and selectivities (H2S/CO2) of new absorbents and iii)the influence of low concentration admixtures, e.g., Fe, Ni and V metals, on the CO2 capture efficiency. The PhD student will acquire valuable experience of industrial area life because he/she will be able independently act in the Unipetrol premises, will cooperate with industrial research department UniCRE and will find here both well-equipped laboratories and experienced consultants.
Development principles: 1. Seznamte se s procesy absorpce doprovázené chemickou reakcí a se způsoby jejich matematického popisu.
2. Proveďte literární rešerši zaměřenou na moderní absorpční roztoky pro vypírky CO2 a jejich vlastnosti.
3. Seznamte se technologií provozovanou v podniku Unipetrol Litvínov a prostudujte dostupnou firemní literaturu tuto technologii popisující
4. Podle konkrétních požadavků průmyslového partnera a experimentálních možností všech zůčatněných pracovišť proveďte experimentální práce vedoucí k vytčeným cílům (viz anotace)
5. Na základě literárních zjištění a výsledků vlastní experimentální práce navrhněte úpravy provozu tlakových vypírek CO2
Faculty of Chemical Engineering

CO2 capture. Industrial process optimization.

Moucha Tomáš, prof. Dr. Ing. ( mou...@vscht.cz)
CO2 capture belongs to frequent industrial needs, both in the cases of low concentration CO2 removal from waste gases and in the cases of main process streams, e.g., in hydrogen production, when high amount/concentration is to be removed. Just the last example represents the process, which the PhD thesis will be focused to. In the premises of Unipetrol company, the CO2 capture unit is presumed to be permanently optimized. In accordance with the needs of the industrial partner, the experimental research goals will involve i) durability / degradability of the solution currently used in the process, ii)absorption efficiencies and selectivities (H2S/CO2) of new absorbents and iii)the influence of low concentration admixtures, e.g., Fe, Ni and V metals, on the CO2 capture efficiency. The PhD student will acquire valuable experience of industrial area life because he/she will be able independently act in the Unipetrol premises, will cooperate with industrial research department UniCRE and will find here both well-equipped laboratories and experienced consultants.
Development principles: 1. Get familiar with the principles of the processes of absorption accompanied by chemical reaction and with their mathematical descriptions.
2. Perform the literary research aimed to modern absorption liquids used in CO2 capture and their properties.
3. Get familiar with the technology used in Unipetrol Litvínov and study an available firm literature describing it.
4. According to the specific requirements of the industrial partner and the experimental possibilities of all registered workplaces, perform experimental work leading to the set goals (see annotations).
5. Based on the literature findings and on the results of your own experimental results, propose modifications to the operation of CO2 pressure washers.
Faculty of Chemical Engineering

Design and application of supra-lipidic structures

Štěpánek František, prof. Ing. Ph.D. ( Fra...@vscht.cz)
Lizoňová Denisa, Ing. ( den...@gmail.com)
The gastro-intestinal transit, emulsification, digestion and absorption of lipidic components from food is crucial not only from the nutritional point of view but also for the dissolution and absorption of many drugs, and therefore their bioavailability. An increasing number of active pharmaceutical ingredients (APIs) that enter the drug development process are highly lipophilic, which makes their bioavailability susceptible to patient-specific dietary habits and often leads to undesired phenomena such as positive food effect. For some APIs, the bioavailability can be up to five times higher when taken on a full stomach compared to bioavailability in the fasted state. The aim of this project is to develop a formulation platform that would make the dissolution, absorption and pharmacokinetics of lipophilic APIs independent of food intake, while not containing a large amount of lipids in the formulation itself. The idea is to create particles that “look like lipids” on the outside but their volume contains predominantly the API or other excipients. Such structures can include e.g. drug suspensions encapsulated in giant liposomes or their aggregates, drug nanocrystals coated by a phospholipid monolayer, or drug-loaded mesoporous silica particles encapsulated within a lipid bi-layer. These elementary structures can also be combined, carrying e.g. several different APIs, functional excipients for absorption enhancement, or pH modifiers that can further reduce patient-to-patient variability.

Synthesis and Production of Drugs - Drugs and Biomaterials

Faculty of Chemical Engineering

Design and application of supra-lipidic structures

Štěpánek František, prof. Ing. Ph.D. ( Fra...@vscht.cz)
Lizoňová Denisa, Ing. ( den...@gmail.com)
The gastro-intestinal transit, emulsification, digestion and absorption of lipidic components from food is crucial not only from the nutritional point of view but also for the dissolution and absorption of many drugs, and therefore their bioavailability. An increasing number of active pharmaceutical ingredients (APIs) that enter the drug development process are highly lipophilic, which makes their bioavailability susceptible to patient-specific dietary habits and often leads to undesired phenomena such as positive food effect. For some APIs, the bioavailability can be up to five times higher when taken on a full stomach compared to bioavailability in the fasted state. The aim of this project is to develop a formulation platform that would make the dissolution, absorption and pharmacokinetics of lipophilic APIs independent of food intake, while not containing a large amount of lipids in the formulation itself. The idea is to create particles that “look like lipids” on the outside but their volume contains predominantly the API or other excipients. Such structures can include e.g. drug suspensions encapsulated in giant liposomes or their aggregates, drug nanocrystals coated by a phospholipid monolayer, or drug-loaded mesoporous silica particles encapsulated within a lipid bi-layer. These elementary structures can also be combined, carrying e.g. several different APIs, functional excipients for absorption enhancement, or pH modifiers that can further reduce patient-to-patient variability.

Faculty of Chemical Engineering

Development and application of a digital twin bioreactor model for modelling of production of biopharmaceuticals

Šoóš Miroslav, prof. Ing. Ph.D. ( Mir...@vscht.cz)
Production of biopharmaceuticals is typically done in a stirred and sparged bioreactors, which combine a fluid flow and microorganism metabolism to produce final product with desired amount and quality. However, often interplay between poor mixing, low dissolved oxygen concentrations, high amount of CO2 and hydrodynamic stress negatively affect the cell behavior resulting in a lower product amount and decreased product quality. In this project we will use recently development CFD model of stirred and sparged bioreactor capable to predict hydrodynamic stress and mixing time and extend it to descritpion of dissolved O2 and CO2 concentrations, uptake of nutrients and release of metabolic products. Information about the fluid flow will be combined with a hybrid model describing detailed microbial metabolism. Tuning of model parameters and its testing will be done against experimental data measured in fermenters of various sizes. Once validated, developed model will be used to test and propose modifications of existing fermenters to optimize the fermentation process.
Faculty of Chemical Engineering

Development and application of a digital twin bioreactor model for modelling of production of biopharmaceuticals

Šoóš Miroslav, prof. Ing. Ph.D. ( Mir...@vscht.cz)
Production of biopharmaceuticals is typically done in a stirred and sparged bioreactors, which combine a fluid flow and microorganism metabolism to produce final product with desired amount and quality. However, often interplay between poor mixing, low dissolved oxygen concentrations, high amount of CO2 and hydrodynamic stress negatively affect the cell behavior resulting in a lower product amount and decreased product quality. In this project we will use recently development CFD model of stirred and sparged bioreactor capable to predict hydrodynamic stress and mixing time and extend it to descritpion of dissolved O2 and CO2 concentrations, uptake of nutrients and release of metabolic products. Information about the fluid flow will be combined with a hybrid model describing detailed microbial metabolism. Tuning of model parameters and its testing will be done against experimental data measured in fermenters of various sizes. Once validated, developed model will be used to test and propose modifications of existing fermenters to optimize the fermentation process.
Faculty of Chemical Engineering

Development of nanoparticles for drug delivery in wound healing

Šoóš Miroslav, prof. Ing. Ph.D. ( Mir...@vscht.cz)
Main focus of this project is to synthesize multifunctional vesicles loaded with hydrophilic and hydrophobic drugs to be used in wound healing. Since these particles can be used directly or as an intermediate during skin dressing their detailed characterization and colloidal stability will be essential. Student will study the impact of vesicle composition (primary system will be surfactant and cholesterol forming niosomes) and method of preparation on the size and properties of the formed nano carriers as well as drug encapsulation efficiency. Once drug will be loaded into vesicles the release kinetics will be measured as a function of molecular weight of used surfactants and ionic strength. Quality of the prepared samples will be characterized by combination of analytical techniques including 3D modulated DLS, Depolarized DLS, static light scattering, optical video microscopy combined with image analysis and cryo-TEM. While batch production mode is simple to realize, part of the project will be also preparation of multifunctional vesicles in microfluidic systems and compare their properties with batch production method.
Faculty of Chemical Engineering

Development of nanoparticles for drug delivery in wound healing

Šoóš Miroslav, prof. Ing. Ph.D. ( Mir...@vscht.cz)
Main focus of this project is to synthesize multifunctional vesicles loaded with hydrophilic and hydrophobic drugs to be used in wound healing. Since these particles can be used directly or as an intermediate during skin dressing their detailed characterization and colloidal stability will be essential. Student will study the impact of vesicle composition (primary system will be surfactant and cholesterol forming niosomes) and method of preparation on the size and properties of the formed nano carriers as well as drug encapsulation efficiency. Once drug will be loaded into vesicles the release kinetics will be measured as a function of molecular weight of used surfactants and ionic strength. Quality of the prepared samples will be characterized by combination of analytical techniques including 3D modulated DLS, Depolarized DLS, static light scattering, optical video microscopy combined with image analysis and cryo-TEM. While batch production mode is simple to realize, part of the project will be also preparation of multifunctional vesicles in microfluidic systems and compare their properties with batch production method.

Synthesis and Production of Drugs - Drugs and Biomaterials

Faculty of Chemical Engineering

Development of nanoparticles for drug delivery in wound healing

Šoóš Miroslav, prof. Ing. Ph.D. ( Mir...@vscht.cz)
Main focus of this project is to synthesize multifunctional vesicles loaded with hydrophilic and hydrophobic drugs to be used in wound healing. Since these particles can be used directly or as an intermediate during skin dressing their detailed characterization and colloidal stability will be essential. Student will study the impact of vesicle composition (primary system will be surfactant and cholesterol forming niosomes) and method of preparation on the size and properties of the formed nano carriers as well as drug encapsulation efficiency. Once drug will be loaded into vesicles the release kinetics will be measured as a function of molecular weight of used surfactants and ionic strength. Quality of the prepared samples will be characterized by combination of analytical techniques including 3D modulated DLS, Depolarized DLS, static light scattering, optical video microscopy combined with image analysis and cryo-TEM. While batch production mode is simple to realize, part of the project will be also preparation of multifunctional vesicles in microfluidic systems and compare their properties with batch production method.

Faculty of Chemical Engineering

Development of scaling-up methods of industrial mechanically agitated reactors

Moucha Tomáš, prof. Dr. Ing. ( mou...@vscht.cz)
Fermenters or, in general, mechanically agitated aerated vessels are frequently used in industry for the intensification of gas-liquid mass transfer, especially in the case of a low gas to liquid volume ratio. Industrial processes as aerobic fermentations, hydrogenations and chlorinations can serve as examples of their application. In many processes the gas-liquid interfacial mass transfer becomes the rate determining step, so the volumetric mass transfer coefficient becomes the key parameter in the design. Mass transfer laboratory pays many year effort to gas-liquid mass transfer measurement in mechanically agitated gas-liquid dispersions with the aim to formulate the scaling-up rules for industrial vessels design. In the frame of this research an extensive experimental work has been done using various batch types (coalescent, non-coalescent, viscous) and for various impeller types (from purely axially pumping to purely radially pumping ones) and their combinations. After collecting large data series in laboratory scale vessels, experimental work continues using pilot-plant vessel equipped with a modern computer controlled regulation and data acquisition system in the form used also in industry. The aim of the PhD work is to collect the transport characteristics (impeller power, gas hold-up and volumetric mass transfer coefficient, kLa) measured in the pilot-plant vessel using various types of impellers (e.g., Rushton Turbine, Lightnin, Techmix, Pitched Blade impellers). The experimental research will be now focused to the transport characteristics in viscous batch and in the presence of solid particles. Both high viscosity and solid particles presence are typical features of industrial fermentation broths. Based both on the laboratory data and on the pilot-plant data the scaling-up rules will be formulated, which will be employable for industrial gas-liquid contactors design. A PhD student will get acquainted with the design methods of other gas-liquid and vapour-liquid processes as well, because he/she will work in the team dealing also with the absorption columns, distillation columns and ejector bubble columns design. More info: Tomáš Moucha, UCT building B, ground floor, room No. T02a, phone: 220 443 299, e-mail: mouchat@vscht.cz
Development principles: 1. Get familiar with the methodology of industrial gas-liquid contactors (GLC) design and with the basics of an interfacial mass transfer.
2. Get familiar with the transport characteristics used in industrial GLC design and study the interfacial mass transfer theories
3. Learn the experimental techniques to determine the transport characteristics needed in industrial GLC design and get familiar with the evaluation models
4. Build a plan of experiments and evaluation procedures to obtain original results complementary with those measured on the workplace earlier
5. With the use of the database already developed on the workplace, expand the design methodology of GLC, for example, by liquid viscosity effect

Synthesis and Production of Drugs - Drugs and Biomaterials

Faculty of Chemical Engineering

Development of 3D cell cultures for the evaluation of drug delivery systems

Štěpánek František, prof. Ing. Ph.D. ( Fra...@vscht.cz)

Faculty of Chemical Engineering

Development of 3D cell cultures for the evaluation of drug delivery systems

Štěpánek František, prof. Ing. Ph.D. ( Fra...@vscht.cz)
Faculty of Chemical Engineering

Effect of liquid physical properties on the mass transfer in various types of bioreactors

Zedníková Mária, Ing. Ph.D. ( zed...@vscht.cz)
Moucha Tomáš, prof. Dr. Ing. ( mou...@vscht.cz)
The production of new products in the field of biotechnology and pharmacy is often associated with a continuous change in the physical properties of liquids during the fermentation process. The physical properties of the batch mainly affect the mass transfer between gas and liquid and therefore they play a key role in the design of the bioreactor. The aim of the doctoral thesis is to study the mass transfer depending on the physical properties of used liquid media (viscosity, presence of various salts and surfactants) in the three types of the most commonly used bioreactors.
The work is intended as the cooperation of ICPF of the CAS (supervisor's workplace) and UCT Prague (consultant's workplace) appropriately complementing the second PhD topic offered by the consultant. Both cooperating workplaces are well equipped by necessary facilities i) stirred tank reactor, ii) bubble column and iii) air-lift reactor. All bioreactors are adapted to measure the volumetric mass transfer coefficient by the same methods, therefore the results will be comparable.
Requirements for an applicant: master degree in chemical or mechanical engineering, organic technology, biotechnology etc.; ability for teamwork; systematic and creative approach to scientific problems; interest in experimental work.
Development principles: 1. Prostudujte odbornou literaturu na téma vliv fyzikálních vlastností kapaliny na přenos hmoty v disperzi kapalina-plyn.
2. Osvojte si techniky měření fyzikálních vlastností kapalin a vlastností mezifázového povrchu (dynamické povrchové napětí, viskoelasticita povrchu). Osvojte si techniku měření objemového koeficientu přenosu hmoty dynamickou tlakovou metodou.
3. Proveďte systematická měření objemového koeficientu přestupu hmoty pro definované vsádky ve třech typech bioreaktorů: mechanicky míchaný reaktor, probublávaná kolona a air-lift reaktor.
4. Na základě získaných výsledků vyhodnoťte vliv různých fyzikálních vlastností na přenos hmoty v disperzi kapalina plyn.
Faculty of Chemical Engineering

Effect of liquid physical properties on the mass transfer in various types of bioreactors

Zedníková Mária, Ing. Ph.D. ( zed...@vscht.cz)
Moucha Tomáš, prof. Dr. Ing. ( mou...@vscht.cz)
The production of new products in the field of biotechnology and pharmacy is often associated with a continuous change in the physical properties of liquids during the fermentation process. The physical properties of the batch mainly affect the mass transfer between gas and liquid and therefore they play a key role in the design of the bioreactor. The aim of the doctoral thesis is to study the mass transfer depending on the physical properties of used liquid media (viscosity, presence of various salts and surfactants) in the three types of the most commonly used bioreactors.
The work is intended as the cooperation of ICPF of the CAS (supervisor's workplace) and UCT Prague (consultant's workplace) appropriately complementing the second PhD topic offered by the consultant. Both cooperating workplaces are well equipped by necessary facilities i) stirred tank reactor, ii) bubble column and iii) air-lift reactor. All bioreactors are adapted to measure the volumetric mass transfer coefficient by the same methods, therefore the results will be comparable.
Requirements for an applicant: master degree in chemical or mechanical engineering, organic technology, biotechnology etc.; ability for teamwork; systematic and creative approach to scientific problems; interest in experimental work.
Development principles: 1. Perform the literary research on the topic dealing with the effect of liquid physical properties on the mass transfer in gas-liquid dispersion..
2. Learn the measurement techniques of liquid physical properties and of interfacial properties (dynamic surface tension, interfacial viscoelasticity). Learn the measurement technique of volumetric mass transport coefficient by dynamic pressure method.
3. Perform the systematic measurements of volumetric mass transport coefficient for defined liquids in three types of bioreactors: stirred tank reactor, bubble column and air-lift reactor.
4. Based on the obtained results, evaluate the effect of various liquid properties on the mass transfer in the gas-liquid dispersion.

Synthesis and Production of Drugs - Drugs and Biomaterials

Faculty of Chemical Engineering

Erosion-controlled drug release from super-placebo tablets

Štěpánek František, prof. Ing. Ph.D. ( Fra...@vscht.cz)
The rate of drug release from a pharmaceutical tablet is one of its most important quality attributes. As an ever-increasing number of Active Pharmaceutical Ingredients (APIs) are developed in alternative solid-state forms such as metastable polymorphs, co-crystals or amorphs, it is desirable to control the rate of drug release by the properties of the tablet matrix rather than by the properties of the API itself. The aim of this project is to explore the so-called “super-placebo” concept, i.e. tablets that erode in a defined way which is independent of the API they contain. The project will systematically explore the relationship between the rate of tablet erosion, the proportion of soluble and insoluble excipients (e.g. mannitol, microcrystalline cellulose), and the manufacturing process parameters (e.g. compaction pressure). The ability to control drug release rate will be demonstrated using several real-world APIs. Advanced instrumental methods such as Magnetic Resonance Imaging, x-ray micro CT and high-speed video-imaging will be used in order to gain a deep understanding of the underlying tablet erosion mechanisms.

Faculty of Chemical Engineering

Erosion-controlled drug release from super-placebo tablets

Štěpánek František, prof. Ing. Ph.D. ( Fra...@vscht.cz)
The rate of drug release from a pharmaceutical tablet is one of its most important quality attributes. As an ever-increasing number of Active Pharmaceutical Ingredients (APIs) are developed in alternative solid-state forms such as metastable polymorphs, co-crystals or amorphs, it is desirable to control the rate of drug release by the properties of the tablet matrix rather than by the properties of the API itself. The aim of this project is to explore the so-called “super-placebo” concept, i.e. tablets that erode in a defined way which is independent of the API they contain. The project will systematically explore the relationship between the rate of tablet erosion, the proportion of soluble and insoluble excipients (e.g. mannitol, microcrystalline cellulose), and the manufacturing process parameters (e.g. compaction pressure). The ability to control drug release rate will be demonstrated using several real-world APIs. Advanced instrumental methods such as Magnetic Resonance Imaging, x-ray micro CT and high-speed video-imaging will be used in order to gain a deep understanding of the underlying tablet erosion mechanisms.
Faculty of Chemical Engineering

Experimental study of morphology formation for polymers and polymer films

Kosek Juraj, prof. Dr. Ing. ( Jur...@vscht.cz)
Zubov Alexandr, Ing. Ph.D. ( Ale...@vscht.cz)
This PhD project will focus on the morphology evolution in selected polymeric systems: (i) semi-crystalline polyolefins including high-impact polypropylene, (ii) films formed from polymer latexeses. Morphology of polymers predetermines their application (i.e., mechanical, optical and transport) properties. The goal of this project is the experimentally-based analysis of the detailed mechanism of morphology formation by using of numerous visualization techniques (AFM, SEM/TEM, micro-CT, confocal Raman, SAXS, DSC, TD-NMR, ...).

One of difficult tasks is the morphology visualization of semi-crystalline polyolefins (lamellae ~ 10 nm) organized into super-structures (~ 100 až 1000 nm). The difficulty of this task is given by sometimes ambiguous interpretation of various visualization techniques and by not yet well-developed methodology of morphology descriptors. The new problematics will be the formation of films from polymer latexes by processes of: (i) evaporation, concentration and ordering of colloid particles, (ii) particle deformation, (iii) difusion of polymer chains. The goal is the morphology characterization in various stages between the original latex and the final compact film.

Polymer morphology is the consequence of various processes driven by phase-separation thermodynamics including crystallization, by the kinetics of crystallization, by diffusion and osmotic processes, by interactions involving capillary effects and surface tension, and by electrostatic and van der Waals interactions. Consequences of these and further morphology-forming processes can be correctly interpreted only by both theoretically and practically prepared PhD student.

The results of this challenging PhD project will be not only high-quality papers, but also expert in many fields of polymer and material engineering. Experiences gained during this project will be broadly applicable also for other polymers, metail and wood materials, biology systems, ceramics, food and personal care products etc.

Info: phone +420 220 44 3296, room B-145, e-mail jkk@vscht.cz, web http://kosekgroup.cz
Faculty of Chemical Engineering

Fluid separations in the modern era challenges

Rejl František, doc.Ing. Ph.D. ( r...@vscht.cz)
Fluid separation processes are widely used and time proven large-capacity separation processes (eg absorption, extraction, distillation). Many new technologies (electric cars, new light sources, geothermal energy sources, etc.) are currently gaining market share and corresponding need for recycling and waste management leads to the necessity of the development of the new high capacity separation technologies. The work should lead to the development/refinement of the selected separation technology in cooperation with an industrial partner.
Faculty of Chemical Engineering

Formation of Micro and Nanostructured Heat Insulation Materials

Kosek Juraj, prof. Dr. Ing. ( Jur...@vscht.cz)
Zubov Alexandr, Ing. Ph.D. ( Ale...@vscht.cz)
The aim of this project is the optimization of insulation properties of available materials and the development of a new generation of insulation materials based on experimental and theoretical knowledge about formation of micro- and nanostructured materials. These materials would dramatically lower the energy consumption for heating and conditioning of buildings.

Micro- and nanostructured materials can be prepared in different ways. The Ph.D. student will focus mainly on the following preparation methods: (i) laser and (ii) pressure induced foaming, and (iii) thermally induced phase separation. The laser induced foaming is a novel method, which enables to observe early stages of formation of micro- and nanostructured materials. The knowledge about the formation of these materials is insufficient and therefore micro- and nanostructured material with several-fold improved heat insulation properties are not still mass produced.

Pressure induced foaming enables to optimize current materials and it uses supercritical CO2 instead of common organic blowing agents. The method of thermally induced phase separation enables to prepare different structure of micro- and nanostructured materials. This opens new application possibilities for micro- and nanostructured materials.

Ph.D. student will be supported by well-equipped laboratories for structural analysis of materials (optical microscope, micro-CT, SEM, AFM, Hg porosimeter, He pycnometer, BET, confocal Raman) including devices to study sorption, transport and heat insulation properties. The student will cooperate with the Institute of Chemical Process Fundamentals of the Czech Academy of Science and New Technologies Research Centre – University of West Bohemia, and will be sent for a research stay into one of our European cooperation centres. This work is supported with grants and industrial cooperations.

Info: phone 220 44 3296, office B-145, e-mailjkk@vscht.cz, web http://kosekgroup.cz

Synthesis and Production of Drugs - Drugs and Biomaterials

Faculty of Chemical Engineering

Formulation and bioavailability of natural poly-actives

Štěpánek František, prof. Ing. Ph.D. ( Fra...@vscht.cz)
Current paradigm in pharmaceutical drug development and its regulatory environment is based on the concept of Active Pharmaceutical Ingredient (API) as a well-defined single molecular entity that is contained in the dosage form at a precise quantity and chemical purity. Although rational in many ways, this approach is rather different from evolution-proof substances found in Nature. The main drawback is single-API medicines is the development of drug resistance over historically extremely short time periods (only a few decades), which is problematic not only in the area of antibiotics but also in cancer treatment, anti-fungal and various anti-parasitic drugs that gradually lose their effectiveness. In contrast, there are examples of natural systems that maintain their efficacy for many millennia. Perhaps the most prominent example of such material is bee propolis. Chemically, propolis is a mixture of several hundred chemical species with location- and season-dependent composition, which would completely disqualify it as a registered medicinal substance. However, it is exactly this variable multi-component character that makes is so robust and durable, not giving pathogens a chance to develop resistance. Propolis contains both water-soluble and water-insoluble components and is typically applied as ethanol dispersion only for surface treatment. The aim of this project is to explore formulation approaches that could enable oral administration of propolis and ensure its safety and bioavailability. The project is multidisciplinary and will include not only formulation and analytical work, but also in vitro and in vivo testing of biological efficacy.

Faculty of Chemical Engineering

Formulation and bioavailability of natural poly-actives

Štěpánek František, prof. Ing. Ph.D. ( Fra...@vscht.cz)
Current paradigm in pharmaceutical drug development and its regulatory environment is based on the concept of Active Pharmaceutical Ingredient (API) as a well-defined single molecular entity that is contained in the dosage form at a precise quantity and chemical purity. Although rational in many ways, this approach is rather different from evolution-proof substances found in Nature. The main drawback is single-API medicines is the development of drug resistance over historically extremely short time periods (only a few decades), which is problematic not only in the area of antibiotics but also in cancer treatment, anti-fungal and various anti-parasitic drugs that gradually lose their effectiveness. In contrast, there are examples of natural systems that maintain their efficacy for many millennia. Perhaps the most prominent example of such material is bee propolis. Chemically, propolis is a mixture of several hundred chemical species with location- and season-dependent composition, which would completely disqualify it as a registered medicinal substance. However, it is exactly this variable multi-component character that makes is so robust and durable, not giving pathogens a chance to develop resistance. Propolis contains both water-soluble and water-insoluble components and is typically applied as ethanol dispersion only for surface treatment. The aim of this project is to explore formulation approaches that could enable oral administration of propolis and ensure its safety and bioavailability. The project is multidisciplinary and will include not only formulation and analytical work, but also in vitro and in vivo testing of biological efficacy.

Chemical and Process Engineering

Faculty of Chemical Engineering

Gas - Liquid Mass Transfer. Experimental comparison of various apparatuses performance.

Moucha Tomáš, prof. Dr. Ing. ( mou...@vscht.cz)
The volumetric mass transfer coefficient (kLa) plays a crucial role in industrial design in the case of the process controlled by gas–liquid mass transfer. Prediction of kLa is nowadays mostly based on literature correlations. Our research goal is to establish suitable kLa correlations for different types of devices that would be based on the experimental dataset. The PhD thesis aim at the comparison of various gas-liquid contactor types from the viewpoint of their mass transfer efficiency. The suitable correlations will be developed that would be viable for mechanically agitated gas–liquid contactors and also for pneumatically agitated gas–liquid contactors such as airlift reactor.
Development principles: 1. Get familiar with mathematical descriptions of a gas-liquid interfacial mass transfer including mass balances of various gas-liquid contactor types
2. Study mathematical models of the mass transport which enable the determination of gas-liquid mass transfer coefficients from experimental data
3. Get to know the experimental apparatuses available at UNIPA in the working group of prof. Brucato and prof. Scargiali as well as those at UCT Prague in the Mass Transfer Laboratory
4. Build the experimental plan which will ensure obtaining experimental results to compare the effectivity of individual apparatuses according to the criteria selected
5. Theoretical findings, experimental procedures and results as well as the conclusions formulated write in the dissertation
Faculty of Chemical Engineering

Gas - Liquid Mass Transfer. Experimental comparison of various apparatuses performance.

Moucha Tomáš, prof. Dr. Ing. ( mou...@vscht.cz)
The volumetric mass transfer coefficient (kLa) plays a crucial role in industrial design in the case of the process controlled by gas–liquid mass transfer. Prediction of kLa is nowadays mostly based on literature correlations. Our research goal is to establish suitable kLa correlations for different types of devices that would be based on the experimental dataset. The PhD thesis aim at the comparison of various gas-liquid contactor types from the viewpoint of their mass transfer efficiency. The suitable correlations will be developed that would be viable for mechanically agitated gas–liquid contactors and also for pneumatically agitated gas–liquid contactors such as airlift reactor.
Development principles: 1. Seznamte se s popisy mezifázového transportu hmoty mezi plynem a kapalinou včetně bilancí různých typů kontaktorů kapalina - plyn
2. Prostudujte matematické popisy procesu sdílení hmoty, které umožňují stanovení koeficientů přestupu hmoty z experimentálních dat
3. Seznamte se s dostupnými experimentálními zařízeními na UNIPA v pracovní skupině prof. Brucato a prof. Scargiali a na VŠCHT v Laboratoři SH
4. Sestavte plán experimentů, které poskytnou výsledky umožňující porovnání efektivity jednotlivých zařízení podle zvolených kritérií
5. Teoretické poznatky, experimentální postupy a výsledky sepište společně se závěry do podoby dizertační práce

Faculty of Chemical Engineering

High-throughput development and continuous manufacturing of SMEDD systems

Štěpánek František, prof. Ing. Ph.D. ( Fra...@vscht.cz)
Self micro-emulsifying drug delivery systems (SMEDDS) are formulations that spontaneously form a mini- or micro-emulsion upon contact with water. They typically contain the active pharmaceutical ingredient (API), a mixture of oils or low-melting lipids in which the API is soluble, and one or more surfactants and co-surfactants. SMEDDS are complex ternary or higher-order mixtures whose phase behaviour and properties are notoriously difficult to predict at present. Therefore, the development of SMEDDS is to a large extent an empirical process. Due to a large number of formulation components and their possible ratios, it is rarely possible to completely cover the entire design space, which may lead to sub-optimum formulations or even a false rejection of a particular API as non-formulatable. The aim of this project is to construct a device and develop a methodology for automatic combinatorial screening of SMEDDS formulations and their continuous manufacturing based on the so-called liquid marbles. The project will build on a recently developed patented device called “Marblemat” and extend its capabilities towards combinatorial mixing of formulation components and serial production of liquid marbles with systematically varying composition. Simultaneously, capability for high-throughput testing of the formulation properties such as mechanical strength, temperature stability and dissolution properties will be implemented and demonstrated on several industry-relevant APIs.

Synthesis and Production of Drugs - Drugs and Biomaterials

Faculty of Chemical Engineering

High-throughput development and continuous manufacturing of SMEDD systems

Štěpánek František, prof. Ing. Ph.D. ( Fra...@vscht.cz)
Self micro-emulsifying drug delivery systems (SMEDDS) are formulations that spontaneously form a mini- or micro-emulsion upon contact with water. They typically contain the active pharmaceutical ingredient (API), a mixture of oils or low-melting lipids in which the API is soluble, and one or more surfactants and co-surfactants. SMEDDS are complex ternary or higher-order mixtures whose phase behaviour and properties are notoriously difficult to predict at present. Therefore, the development of SMEDDS is to a large extent an empirical process. Due to a large number of formulation components and their possible ratios, it is rarely possible to completely cover the entire design space, which may lead to sub-optimum formulations or even a false rejection of a particular API as non-formulatable. The aim of this project is to construct a device and develop a methodology for automatic combinatorial screening of SMEDDS formulations and their continuous manufacturing based on the so-called liquid marbles. The project will build on a recently developed patented device called “Marblemat” and extend its capabilities towards combinatorial mixing of formulation components and serial production of liquid marbles with systematically varying composition. Simultaneously, capability for high-throughput testing of the formulation properties such as mechanical strength, temperature stability and dissolution properties will be implemented and demonstrated on several industry-relevant APIs.

Faculty of Chemical Engineering

Characterization and modelling of dispersion systems with variable viscosity

Šoóš Miroslav, prof. Ing. Ph.D. ( Mir...@vscht.cz)
The goal of this project is to characterize and model systems where viscosity of the dispersed phase is rising during the process. Typical examples are emulsification, suspension polymerization or spherical agglomeration. The student will start with simplified system composed of two liquid phases with various viscosities, which will be analyzed by on-line sensors providing information about the droplets sizes. Experimental activity will cover both batch as well as continuous operation modes. Collected data will be consequently used to develop engineering model based on computational fluid dynamic of the fluid flow coupled with population balances to describe coalescence and breakup of dispersed phase for various levels of dispersed phase viscosity. An extension of this activity will be process of spherical agglomeration where dispersed phase will contain particles (nanoparticles or crystals), which can undergo agglomeration and thus increasing the viscosity of the dispersed phase. Developed model will be validated against experimental data collected at various scales or operating conditions.
Faculty of Chemical Engineering

Characterization and modelling of dispersion systems with variable viscosity

Šoóš Miroslav, prof. Ing. Ph.D. ( Mir...@vscht.cz)
The goal of this project is to characterize and model systems where viscosity of the dispersed phase is rising during the process. Typical examples are emulsification, suspension polymerization or spherical agglomeration. The student will start with simplified system composed of two liquid phases with various viscosities, which will be analyzed by on-line sensors providing information about the droplets sizes. Experimental activity will cover both batch as well as continuous operation modes. Collected data will be consequently used to develop engineering model based on computational fluid dynamic of the fluid flow coupled with population balances to describe coalescence and breakup of dispersed phase for various levels of dispersed phase viscosity. An extension of this activity will be process of spherical agglomeration where dispersed phase will contain particles (nanoparticles or crystals), which can undergo agglomeration and thus increasing the viscosity of the dispersed phase. Developed model will be validated against experimental data collected at various scales or operating conditions.
Faculty of Chemical Engineering

Influence of composite particle structure for targeted drug delivery

Tokárová Viola, Ing. Ph.D. ( vio...@vscht.cz)
The encapsulation of active substances in microscopic carriers finds its practical application in the food industry, cosmetics and pharmacy. The active substance is thus protected from the environment and can be delivered to the target site of action (eg: damaged tissue, tumour cells). The aim of this work is to prepare particles with diverse architecture and topography (eg: core-shell, particles with microtopography, fibrous structure) and to investigate the influence of particle architecture on release kinetics of encapsulated active ingredient. Methods of spray drying, encapsulation and microfluidics will be used for the preparation of composite particle systems in this work. The active ingredient will be selected with respect to the biomedical application.
Faculty of Chemical Engineering

Influence of composite particle structure for targeted drug delivery

Tokárová Viola, Ing. Ph.D. ( vio...@vscht.cz)
The encapsulation of active substances in microscopic carriers finds its practical application in the food industry, cosmetics and pharmacy. The active substance is thus protected from the environment and can be delivered to the target site of action (eg: damaged tissue, tumour cells). The aim of this work is to prepare particles with diverse architecture and topography (eg: core-shell, particles with microtopography, fibrous structure) and to investigate the influence of particle architecture on release kinetics of encapsulated active ingredient. Methods of spray drying, encapsulation and microfluidics will be used for the preparation of composite particle systems in this work. The active ingredient will be selected with respect to the biomedical application.
Faculty of Chemical Engineering

KLa - shear stress coupling to design fermenters better

Moucha Tomáš, prof. Dr. Ing. ( mou...@vscht.cz)
In fermentation technologies, mechanically agitated aerated vessels are frequently used. In cases of aerobic fermentations, the Oxygen Uptake Rate - OUR is frequently used as the important design parameter. This means that the gas-liquid mass transfer controlled process is considered and the volumetric mass transfer coefficient - kLa is taken as the most important parameter. The practice shows, however, that the impellers with lower Power number (which means lower turbulence intensity and lower kLa) often ensure higher bioprocess efficiency than those with high Power number (which means higher turbulence intensity and higher kLa). The explanation is brought by the fact that microorganisms/biomass might be damaged by the high turbulence intensity as explained further. The turbulence intensity is proportional to shear stresses occuring in the mechanically agitated fermentation batch. A high shear stress may "cut" the microorganisms, which stop producing their primary product then. The aim of the PhD thesis is to measure the quantities proportional to shear stress values at the process conditions of aerobic fermentations and couple them with the kLa values, which are already at disposal in the Mass Transfer Lab database at UCT Prague. This data coupling will enable to develope the highly efficient industrial fermenters design tool.
Development principles: 1. Seznamte se s popisem transportu hmoty mezi plynem a kapalinou v mechanicky míchaných disperzích
2. Seznamte se s popisem lokálních intenzit turbulnce, fluktuačních rychostí a střižných sil v mechanicky míchaných disperzích kapalina-plyn
3. Prostudujte experimentální techniky měření kLa v Laboratoři sdílení hmoty VŠCHT Praha a techniky měření veličin úměrných intenzitě turbulence na TU Berlin.
4. Prostudujte experimetální podmínky použité k získání kLa dat v databázi na VŠCHT Praha a za shodných podmínek proměřte veličiny úměrné intenzitě turbulence metodikami užívanými na TU Berlin
5. Doplňte databázi kLa dat o veličiny použitelné k výpočtu střižných rychlostí a navrhněte způsob využití takto spárovaných veličin pro návrh mechanicky míchaných fermentorů.
Faculty of Chemical Engineering

KLa - shear stress coupling to design fermenters better

Moucha Tomáš, prof. Dr. Ing. ( mou...@vscht.cz)
In fermentation technologies, mechanically agitated aerated vessels are frequently used. In cases of aerobic fermentations, the Oxygen Uptake Rate - OUR is frequently used as the important design parameter. This means that the gas-liquid mass transfer controlled process is considered and the volumetric mass transfer coefficient - kLa is taken as the most important parameter. The practice shows, however, that the impellers with lower Power number (which means lower turbulence intensity and lower kLa) often ensure higher bioprocess efficiency than those with high Power number (which means higher turbulence intensity and higher kLa). The explanation is brought by the fact that microorganisms/biomass might be damaged by the high turbulence intensity as explained further. The turbulence intensity is proportional to shear stresses occuring in the mechanically agitated fermentation batch. A high shear stress may "cut" the microorganisms, which stop producing their primary product then. The aim of the PhD thesis is to measure the quantities proportional to shear stress values at the process conditions of aerobic fermentations and couple them with the kLa values, which are already at disposal in the Mass Transfer Lab database at UCT Prague. This data coupling will enable to develope the highly efficient industrial fermenters design tool.
Development principles: 1. Seznamte se s popisem transportu hmoty mezi plynem a kapalinou v mechanicky míchaných disperzích
2. Seznamte se s popisem lokálních intenzit turbulnce, fluktuačních rychostí a střižných sil v mechanicky míchaných disperzích kapalina-plyn
3. Prostudujte experimentální techniky měření kLa v Laboratoři sdílení hmoty VŠCHT Praha a techniky měření veličin úměrných intenzitě turbulence na TU Berlin.
4. Prostudujte experimetální podmínky použité k získání kLa dat v databázi na VŠCHT Praha a za shodných podmínek proměřte veličiny úměrné intenzitě turbulence metodikami užívanými na TU Berlin
5. Doplňte databázi kLa dat o veličiny použitelné k výpočtu střižných rychlostí a navrhněte způsob využití takto spárovaných veličin pro návrh mechanicky míchaných fermentorů.

Chemical and Process Engineering

Faculty of Chemical Engineering

KLa - shear stress coupling to design fermenters better

Moucha Tomáš, prof. Dr. Ing. ( mou...@vscht.cz)
In fermentation technologies, mechanically agitated aerated vessels are frequently used. In cases of aerobic fermentations, the Oxygen Uptake Rate - OUR is frequently used as the important design parameter. This means that the gas-liquid mass transfer controlled process is considered and the volumetric mass transfer coefficient - kLa is taken as the most important parameter. The practice shows, however, that the impellers with lower Power number (which means lower turbulence intensity and lower kLa) often ensure higher bioprocess efficiency than those with high Power number (which means higher turbulence intensity and higher kLa). The explanation is brought by the fact that microorganisms/biomass might be damaged by the high turbulence intensity as explained further. The turbulence intensity is proportional to shear stresses occuring in the mechanically agitated fermentation batch. A high shear stress may "cut" the microorganisms, which stop producing their primary product then. The aim of the PhD thesis is to measure the quantities proportional to shear stress values at the process conditions of aerobic fermentations and couple them with the kLa values, which are already at disposal in the Mass Transfer Lab database at UCT Prague. This data coupling will enable to develope the highly efficient industrial fermenters design tool.
Development principles: 1. Get familiar with the description of the gas-liquid mass transfer in mechanically agitated dispersions.
2. Get familiar with the descritpion of local turbulence intensities., fluctuation velocities and shear stresses in mechanically agitated gas-liquid disperions.
3. Study experimental techniques of kLa measurement in UCT Mass transfer Laboratory and of the quantities proportional to the local turbulence intensity used at TU Berlin.
4. Study the eperimental conditions used in Mass Transfer Laboratory at UCT Prague to obtain kLa database and then, at the same conditions measure the quantities proportional to local turbulence intensities as used at TU Berlin.
5. Complete the kLa database by the quantities usable to compute shear stresses and suggest the way how to use these coupled data in industrial fermenters design.
Faculty of Chemical Engineering

KLa - shear stress coupling to design fermenters better

Moucha Tomáš, prof. Dr. Ing. ( mou...@vscht.cz)
In fermentation technologies, mechanically agitated aerated vessels are frequently used. In cases of aerobic fermentations, the Oxygen Uptake Rate - OUR is frequently used as the important design parameter. This means that the gas-liquid mass transfer controlled process is considered and the volumetric mass transfer coefficient - kLa is taken as the most important parameter. The practice shows, however, that the impellers with lower Power number (which means lower turbulence intensity and lower kLa) often ensure higher bioprocess efficiency than those with high Power number (which means higher turbulence intensity and higher kLa). The explanation is brought by the fact that microorganisms/biomass might be damaged by the high turbulence intensity as explained further. The turbulence intensity is proportional to shear stresses occuring in the mechanically agitated fermentation batch. A high shear stress may "cut" the microorganisms, which stop producing their primary product then. The aim of the PhD thesis is to measure the quantities proportional to shear stress values at the process conditions of aerobic fermentations and couple them with the kLa values, which are already at disposal in the Mass Transfer Lab database at UCT Prague. This data coupling will enable to develope the highly efficient industrial fermenters design tool.
Development principles: 1. Seznamte se s popisem transportu hmoty mezi plynem a kapalinou v mechanicky míchaných disperzích
2. Seznamte se s popisem lokálních intenzit turbulnce, fluktuačních rychostí a střižných sil v mechanicky míchaných disperzích kapalina-plyn
3. Prostudujte experimentální techniky měření kLa v Laboratoři sdílení hmoty VŠCHT Praha a techniky měření veličin úměrných intenzitě turbulence na TU Berlin.
4. Prostudujte experimetální podmínky použité k získání kLa dat v databázi na VŠCHT Praha a za shodných podmínek proměřte veličiny úměrné intenzitě turbulence metodikami užívanými na TU Berlin
5. Doplňte databázi kLa dat o veličiny použitelné k výpočtu střižných rychlostí a navrhněte způsob využití takto spárovaných veličin pro návrh mechanicky míchaných fermentorů.

Faculty of Chemical Engineering

Liquid-gas ejector as compact and economic reactor

Haidl Jan, Ing. Ph.D. ( jan...@vscht.cz)
Moucha Tomáš, prof. Dr. Ing. ( mou...@vscht.cz)
Faculty of Chemical Engineering

Liquid-gas ejector as compact and economic reactor

Haidl Jan, Ing. Ph.D. ( jan...@vscht.cz)
Faculty of Chemical Engineering

Mathematical modeling of microfluidic devices for separation of racemic

Přibyl Michal, prof. Ing. Ph.D. ( pri...@vscht.cz)
Microfluidic devices are characterized by a large ratio of interfacial area to internal volume. This can be used in chemical separations by extraction or membrane processes. Separation of optically active substances, often important pharmaceutical or food products, at membranes or sorbents with anchored chiral selectors represents a great challenge for chemical engineers. Mathematical modeling can lead to a better understanding of complex processes in such devices and consequently to the design of efficient microfluidic separators.
The main objectives of the PhD project are:
Based on preliminary and available experimental data, a mathematical-physical description of mass and momentum transport in microfluidic devices with anchored chiral selectors will be developed.
Mathematical models of processes on different spatial scales will be created. They will include description of transport of the separated chemicals by diffusion, convection and electromigration.
Models will be analyzed numerically. Parameter values that ensure high separation efficiency and high productivity of the microfluidic system will be searched in the parameter space.
The lab is equipped with modern computers. The participation of the doctoral student in grant projects and active participation in international scientific conferences is expected.
Faculty of Chemical Engineering

Mathematical modeling of microfluidic devices for separation of racemic mixtures

Přibyl Michal, prof. Ing. Ph.D. ( pri...@vscht.cz)
Microfluidic devices are characterized by a large ratio of interfacial area to internal volume. This can be used in chemical separations by extraction or membrane processes. Separation of optically active substances, often important pharmaceutical or food products, at membranes or sorbents with anchored chiral selectors represents a great challenge for chemical engineers. Mathematical modeling can lead to a better understanding of complex processes in such devices and consequently to the design of efficient microfluidic separators. The main objectives of the PhD project are: Based on preliminary and available experimental data, a mathematical-physical description of mass and momentum transport in microfluidic devices with anchored chiral selectors will be developed. Mathematical models of processes on different spatial scales will be created. They will include description of transport of the separated chemicals by diffusion, convection and electromigration. Models will be analyzed numerically. Parameter values that ensure high separation efficiency and high productivity of the microfluidic system will be searched in the parameter space. The lab is equipped with modern computers. The participation of the doctoral student in grant projects and active participation in international scientific conferences is expected.
Faculty of Chemical Engineering

Membrane separation of fermentation primary products

Moucha Tomáš, prof. Dr. Ing. ( mou...@vscht.cz)
In biotechnologies, batch processes are often used, in which living cultures/biomass are used. Metabolites produced by culture are often poisonous and can damage the culture itself, as an example of which can serve ethanol fermentation. In periodic processes, the initial periods including sterilizing, nutrient dosing, etc., used to be time consuming, financially burdening. Therefore, it is desirable to dedicate an effort to develope continuous performance of such processes. One of the operations ensuring the process continualization can be membrane separation. This case brings the necessity of two membranes modules: i) microfiltration to separate solid particles-biomass and ii) pervaporation to separate primary product of fermentation, e.g., ethanol, as mentioned above.
The goal of this work is to experimentally develop two step membarne separation technique, including microfiltration and pervaporation, to be prepared for an interconnection with a fermenter. THe development will be conducted from the viewpoint of chemical engineering. The reached separation parameters (selectivity, permeability) will be investigated in dependency on the process parameters (pressure, flowrate, temperature, feed composition). Chemical engineering quantities (membrain polarization module, mass transfer coefficient,...) will be used to describe these dependencies. At the workplace the new membrane modules are available, which were purchased for the purpose of this development. The PhD student will get familier both with industrial membrane module and with the custom made one.
In addition to being familiar with modern technologies introduced in industry, the PhD student will also work in the team of students and academic staff who are experienced in industrial cooperation. PhD study will prepare the student to obtain either qualified working position in industry or to be able systematically conduct further research from the viewpoint of qualified chemical engineer.
Further information Assoc. Prof. Tomáš Moucha, UCT Prague, building B, room T02, email: tomas.mooucha@vscht.cz
Development principles: 1. Seznamte se s principy membránových separačních procesů, jejich fyzikálními představami a základními matematickými popisy.
2. Proveďte literární rešerši na téma specifik membránových procesů mikrofiltrace a pervaporace se zvláštním zaměřením na popis nežádoucích jevů a způsobů jejich potlačování
3. Seznamte se detailně s konstrukcí a funkcemi membránových modulů pro mikrofiltraci a pervaporaci v Laboratoři sdílení hmoty
4. Proveďte série experimentů v dostatečně širokém záběru provozních parametrů, aby bylo možno zmapovat jejich vliv na klíčové parametry pro posouzení efektivity separace jako například selektivita membrány a kapacita membránového modulu
5. Experimentální výsledky zpracujte do tabulek a grafů, ze kterých lze vysledovat trendy separační účinnosti s provozními parametry a pokuste se tyto trendy popsat matematickými modely, které je možno použít pro optimalizaci provozu studovaných separačních procesů
Faculty of Chemical Engineering

Membrane separation of fermentation primary products

Moucha Tomáš, prof. Dr. Ing. ( mou...@vscht.cz)
In biotechnologies, batch processes are often used, in which living cultures/biomass are used. Metabolites produced by culture are often poisonous and can damage the culture itself, as an example of which can serve ethanol fermentation. In periodic processes, the initial periods including sterilizing, nutrient dosing, etc., used to be time consuming, financially burdening. Therefore, it is desirable to dedicate an effort to develope continuous performance of such processes. One of the operations ensuring the process continualization can be membrane separation. This case brings the necessity of two membranes modules: i) microfiltration to separate solid particles-biomass and ii) pervaporation to separate primary product of fermentation, e.g., ethanol, as mentioned above. The goal of this work is to experimentally develop two step membarne separation technique, including microfiltration and pervaporation, to be prepared for an interconnection with a fermenter. THe development will be conducted from the viewpoint of chemical engineering. The reached separation parameters (selectivity, permeability) will be investigated in dependency on the process parameters (pressure, flowrate, temperature, feed composition). Chemical engineering quantities (membrain polarization module, mass transfer coefficient,...) will be used to describe these dependencies. At the workplace the new membrane modules are available, which were purchased for the purpose of this development. The PhD student will get familier both with industrial membrane module and with the custom made one. In addition to being familiar with modern technologies introduced in industry, the PhD student will also work in the team of students and academic staff who are experienced in industrial cooperation. PhD study will prepare the student to obtain either qualified working position in industry or to be able systematically conduct further research from the viewpoint of qualified chemical engineer. Further information Assoc. Prof. Tomáš Moucha, UCT Prague, building B, room T02, email: tomas.mooucha@vscht.cz
Development principles: 1. Get familiar with the principles of membrane separation processes, their physical concepts and basic mathematical description.
2. Elaborate literature search on the specifics of microfiltration and pervaporation focusing on undesirable phenomena and on the ways of their elimination.
3. Get familiar with the details of construction of microfiltration and pervaporation membrane modules in the Mass Transfer Laboratory.
4. Run a series of experiments using sufficiently wide range of operational conditions to capture the trends in their effect on the separation efficiency, for example, in terms of the membrane selectivity and module capacity.
5. Organize your experimental results into charts and graphs, which will illustrate the trends in separation efficiency with the operational parameters. Try to describe the trends by mathematical models, which could be used in the optimization of separation processes.
Faculty of Chemical Engineering

Microfluidic systems for the synthesis and separation of optically active

Přibyl Michal, prof. Ing. Ph.D. ( pri...@vscht.cz)
Microfluidic reactors and separators are modern devices that represent an alternative to conventional batch and flow systems used in biotechnology. The small spatial scale ensures reproducible reaction conditions and intensive mass and heat transfer. Microfluidic devices generally lack moving parts and allow easy combination of many unit operations such as mixers, separators, reactors. The main objectives of the PhD project are: Study of kinetics of selected enzymatic reactions, which lead to production of optically active chemicals that are used in pharmacy, food industry or synthesis of chemical specialties. Design and fabrication of microfluidic separators with embedded membrane or sorbents with attached chiral selectors for separation of racemic mixtures. Testing of manufactured microfluidic devices for selective separation of selected optically active compounds. Evaluation of the possibility of accelerated transport of optically active substances through membranes by means of an imposed electric field. The lab is equipped with technologies for the production of microfluidic systems, modern measuring instruments and powerful computers. The participation of the doctoral student in grant projects and active participation in international scientific conferences is expected.
Faculty of Chemical Engineering

Microfluidic systems for the synthesis and separation of optically active chemicals

Přibyl Michal, prof. Ing. Ph.D. ( pri...@vscht.cz)
Microfluidic reactors and separators are modern devices that represent an alternative to conventional batch and flow systems used in biotechnology. The small spatial scale ensures reproducible reaction conditions and intensive mass and heat transfer. Microfluidic devices generally lack moving parts and allow easy combination of many unit operations such as mixers, separators, reactors.
The main objectives of the PhD project are:
Study of kinetics of selected enzymatic reactions, which lead to production of optically active chemicals that are used in pharmacy, food industry or synthesis of chemical specialties.
Design and fabrication of microfluidic separators with embedded membrane or sorbents with attached chiral selectors for separation of racemic mixtures.
Testing of manufactured microfluidic devices for selective separation of selected optically active compounds.
Evaluation of the possibility of accelerated transport of optically active substances through membranes by means of an imposed electric field.
The lab is equipped with technologies for the production of microfluidic systems, modern measuring instruments and powerful computers. The participation of the doctoral student in grant projects and active participation in international scientific conferences is expected.
Faculty of Chemical Engineering

Micro-scale mathematical modeling of gas-liquid transport in catalyst pores

Kočí Petr, doc. Ing. Ph.D. ( pet...@vscht.cz)
Isoz Martin, Ing. Ph.D. ( i...@vscht.cz)
The work focuses on the development of advanced mathematical models for simulations of mass transport in multiphase systems gas-liquid indide pores of a solid catalyst, including phase change (evaporation, condensation). The models are developed in the CFD environment OpenFOAM and utilize 3D-reconstructed structures of porous materials obtained from X-ray tomography (XRT) and electron microscopy. The model results are further verified with the available experimental data from lab reactor.
Faculty of Chemical Engineering

Micro-scale mathematical modeling of gas-liquid transport in catalyst pores

Kočí Petr, doc. Ing. Ph.D. ( pet...@vscht.cz)
Isoz Martin, Ing. Ph.D. ( i...@vscht.cz)
The work focuses on the development of advanced mathematical models for simulations of mass transport in multiphase systems gas-liquid indide pores of a solid catalyst, including phase change (evaporation, condensation). The models are developed in the CFD environment OpenFOAM and utilize 3D-reconstructed structures of porous materials obtained from X-ray tomography (XRT) and electron microscopy. The model results are further verified with the available experimental data from lab reactor.
Faculty of Chemical Engineering

Modelling of interactions in colloidal systems by Discrete Element Methods

Kosek Juraj, prof. Dr. Ing. ( Jur...@vscht.cz)
Zubov Alexandr, Ing. Ph.D. ( Ale...@vscht.cz)
Various particulate dispersions in liquid phase, e.g., suspensions or emulsions, are found in many products starting from polymer latexes through personal care up to paints and food products. The first aim of this project is to advance our particle-based computer modelling and address numerous challenges. This will be possible due to on-going parallelization of DEM code allowing to calculate at realistic shear-rates.

Examples of open challenges are: (i) rheology and stability of dispersions stabilized by non-ionic surfactants, (ii) formation of colloidal gels, (iii) extensional rheology of concentrated dispersions, (iv) electrostatic charging of dielectric particle-liquid dispersions, (v) formulation of kernels for population balance modelling, and (vi) application of dispersions in energy-storage applications based on metal-air electrochemical cells.

PhD student will become familiar with not only polymer reaction and material engineering, polymer physics, physical chemistry, fluid mechanics and colloid science, but will also advance his/her mathematical modeling skills. The project will be conducted not only with the research team in our research laboratory, but also with partners from European companies and universities. This project is going to be supported by grant projects and contractual research.

Info: phone +420 220 44 3296, office B-145, e-mail jkk@vscht.cz, web http://kosekgroup.cz
Faculty of Chemical Engineering

Nanostructured biomimetic surfaces with antibacterial effect

Tokárová Viola, Ing. Ph.D. ( vio...@vscht.cz)
Biomimetic materials developed thanks to new technologies have a unique function inspired by the biomaterials and their structure occurring in nature. One of the areas of biomimetics is topographic surfaces with antibacterial property. The subject of the work is the mapping of the topography of natural materials, such as the wings of selected representatives of dragonflies or rose petals and replication of the structures into suitable material that can be applied in biomedicine (e.g., implants). The aim is to focus on nanostructured topography and to characterize both surfaces, natural and replicated, and to test antibacterial property of replicas against different bacterial strains (e.g. infections associated with postoperative medical care).
Faculty of Chemical Engineering

Nanostructured biomimetic surfaces with antibacterial effect

Tokárová Viola, Ing. Ph.D. ( vio...@vscht.cz)
Biomimetic materials developed thanks to new technologies have a unique function inspired by the biomaterials and their structure occurring in nature. One of the areas of biomimetics is topographic surfaces with antibacterial property. The subject of the work is the mapping of the topography of natural materials, such as the wings of selected representatives of dragonflies or rose petals and replication of the structures into suitable material that can be applied in biomedicine (e.g., implants). The aim is to focus on nanostructured topography and to characterize both surfaces, natural and replicated, and to test the antibacterial property of replicas against different bacterial strains (e.g. infections associated with postoperative medical care).

Synthesis and Production of Drugs - Drugs and Biomaterials

Faculty of Chemical Engineering

Naturally sourced particles for drug encapsulation and delivery

Štěpánek František, prof. Ing. Ph.D. ( Fra...@vscht.cz)
Drug encapsulation into a suitable carrier particle is a common method used in situations where it is possible to either modify the surface properties (e.g. powder flowability or dispersibility in water), to protect the encapsulated component from the environment (e.g. enzymatic digestion in the GI tract) or to control the rate of drug release. Several man-made encapsulation processes are known. However, there are also many natural systems that rely on encapsulation – the cell walls of single cell organisms or their spores, natural particles such as pollen, extra-cellular vesicles, or sub-cellular structures such as vacuoles or other organelles. Some of these structures are highly specific in terms of drug diffusion and its selectivity, or in terms of recognition by cells of the immune system e.g. due to specific shape of the presence of immunomodulatory functional groups on the surface. Yeast glucan particles can serve as a prime example. The aim of this inter-disciplinary project is to investigate the potential of several different types of naturally sourced particles in drug formulation and drug delivery. Both cell-wall derived particles and organelle-based particles will be considered. Special attention will be paid to the process of particle extraction and isolation, as well to the drug encapsulation methodology.

Faculty of Chemical Engineering

Naturally sourced particles for drug encapsulation and delivery

Štěpánek František, prof. Ing. Ph.D. ( Fra...@vscht.cz)
Drug encapsulation into a suitable carrier particle is a common method used in situations where it is possible to either modify the surface properties (e.g. powder flowability or dispersibility in water), to protect the encapsulated component from the environment (e.g. enzymatic digestion in the GI tract) or to control the rate of drug release. Several man-made encapsulation processes are known. However, there are also many natural systems that rely on encapsulation – the cell walls of single cell organisms or their spores, natural particles such as pollen, extra-cellular vesicles, or sub-cellular structures such as vacuoles or other organelles. Some of these structures are highly specific in terms of drug diffusion and its selectivity, or in terms of recognition by cells of the immune system e.g. due to specific shape of the presence of immunomodulatory functional groups on the surface. Yeast glucan particles can serve as a prime example. The aim of this inter-disciplinary project is to investigate the potential of several different types of naturally sourced particles in drug formulation and drug delivery. Both cell-wall derived particles and organelle-based particles will be considered. Special attention will be paid to the process of particle extraction and isolation, as well to the drug encapsulation methodology.

Synthesis and Production of Drugs - Drugs and Biomaterials

Faculty of Chemical Engineering

Oleogels for drug delivery

Štěpánek František, prof. Ing. Ph.D. ( Fra...@vscht.cz)
Řehoř Ivan, RNDr. Ph.D. ( REH...@vscht.cz)
Oleogels, like hydrogels, are semi-solid materials that can contain up to 99 % of a liquid, which is solidified by a three-dimensional polymer network. While hydrogels contain as the liquid and the polymers are hydrophilic, oleogels contain oil and an oleophilic polymer network. Many APIs that are poorly soluble in water could potentially be formulated using oleogels and be either directly dissolved in the oil phase or form a particle depot that would dissolve in the oil gradually and act as a longer-lasting reservoir. The aim of this project is to evaluate the suitablitity of selected oleogel formulations for drug delivery applications from the point of view of manufacturability, drug release kinetics, drug stability, and biological compatibility. The application of oleogels will be demonstrated using several selected APIs both in vitro and in vivo.

Faculty of Chemical Engineering

Oleogels for drug delivery

Štěpánek František, prof. Ing. Ph.D. ( Fra...@vscht.cz)
Oleogels, like hydrogels, are semi-solid materials that can contain up to 99 % of a liquid, which is solidified by a three-dimensional polymer network. While hydrogels contain as the liquid and the polymers are hydrophilic, oleogels contain oil and an oleophilic polymer network. Many APIs that are poorly soluble in water could potentially be formulated using oleogels and be either directly dissolved in the oil phase or form a particle depot that would dissolve in the oil gradually and act as a longer-lasting reservoir. The aim of this project is to evaluate the suitablitity of selected oleogel formulations for drug delivery applications from the point of view of manufacturability, drug release kinetics, drug stability, and biological compatibility. The application of oleogels will be demonstrated using several selected APIs both in vitro and in vivo.

Synthesis and Production of Drugs - Drugs and Biomaterials

Faculty of Chemical Engineering

On-line measurement and control of continuous pharmaceutical manufacturing

Štěpánek František, prof. Ing. Ph.D. ( Fra...@vscht.cz)
The manufacturing of pharmaceutical products is typically carried out batch-wise. While this makes sense for products that are manufactured only occasionally in small quantities, batch processes also have several drawbacks. These include excessive dead-times, need for cleaning to avoid cross-contamination, and generally poorer control over the product quality. By switching pharmaceutical manufacturing to a continuous mode, equipment utilisation can be increased theoretically to 100 %, the footprint of the facilities can be substantially reduced, and standard feed-back and feed-forward control schemes applied. A crucial component of continuous manufacturing processes is on-line measurement of key quality attributes such as particle size distribution, composition uniformity of granular blends, or moisture content. Advanced analytical instruments such as Near-Infrared probes can be used for this purpose. The aim of this project is to explore the on-line measurement and control methods for continuous pharmaceutical manufacturing in an industrial setting and combine them with computer simulation tools in order to optimize the overall process robustness and operability.

Faculty of Chemical Engineering

On-line measurement and control of continuous pharmaceutical manufacturing

Štěpánek František, prof. Ing. Ph.D. ( Fra...@vscht.cz)
The manufacturing of pharmaceutical products is typically carried out batch-wise. While this makes sense for products that are manufactured only occasionally in small quantities, batch processes also have several drawbacks. These include excessive dead-times, need for cleaning to avoid cross-contamination, and generally poorer control over the product quality. By switching pharmaceutical manufacturing to a continuous mode, equipment utilisation can be increased theoretically to 100 %, the footprint of the facilities can be substantially reduced, and standard feed-back and feed-forward control schemes applied. A crucial component of continuous manufacturing processes is on-line measurement of key quality attributes such as particle size distribution, composition uniformity of granular blends, or moisture content. Advanced analytical instruments such as Near-Infrared probes can be used for this purpose. The aim of this project is to explore the on-line measurement and control methods for continuous pharmaceutical manufacturing in an industrial setting and combine them with computer simulation tools in order to optimize the overall process robustness and operability.
Faculty of Chemical Engineering

Particle Based Modeling of Diffusion, Rheological, Interfacial and Relaxation Properties of Polymers

Kosek Juraj, prof. Dr. Ing. ( Jur...@vscht.cz)
Zubov Alexandr, Ing. Ph.D. ( Ale...@vscht.cz)
Dissipative particle dynamics (DPD) is a stochastic modeling technique for simulating of simple and complex fluids. It is being used to tackle hydrodynamic time and space scales beyond those available with molecular dynamics. DPD is an off-lattice meso-scale simulation technique considering particles moving in a continuous space, where particles represent whole molecules, segments of molecules or fluid regions, rather than single atoms. DPD gives access to simulation of polymer fluids with volumes up to 100 nm in linear dimension for up to a millisecond.

This PhD project is at intersection of many challenges in chemical and polymer reactor engineering. Examples of these challenging problems are: · Diffusion flux supplying monomer(s) in catalytic polymerization is limited by finite molecular and/or segmental motion and not by constitutive equations analogous to Fick’s law. If this hypothesis is confirmed then it will provide a key to explanation of kinetic data obtained over many decades. And standard textbooks of chemical engineering will be changed. · Rheology of concentrated polymer solutions (e.g., polymer swollen by monomer(s)) is an open problem important to suspension polymerization or foaming of polyurethanes. · Diffusion of polymer segments and interfacial phenomena are a key to understanding of phase separation in individual latex nanoparticles in emulsion copolymerization producing high-value specialty products. · Systematic understanding of penetrant diffusion in polyolefins including its temperature dependence and interplay between diffusion and relaxation dynamics is another problem with a pool of available experimental data but insufficient underlying theories.

PhD student will become familiar with not only polymer reaction and material engineering, polymer physics, physical chemistry, fluid mechanics and colloid science, but will also advance his/her mathematical modeling skills. The project will be conducted not only with the research team in our research laboratory, but also with partners from European companies and universities. This project is going to be supported by grant projects and contractual research.

Info: phone +420 220 44 3296, office B-145, e-mail jkk@vscht.cz, web http://kosekgroup.cz
Faculty of Chemical Engineering

Porous catalytic layers in structured reactors

Kočí Petr, doc. Ing. Ph.D. ( pet...@vscht.cz)
The work focuses on preparation and coating of porous catalytic layers in structured reactors such as honeycomb monoliths, filters, porous membranes and open foams. The aim is to intensify the reactor operation with respect to utilization of the catalytic material and mass and heat tranfer. Industrially relevant processes such as exhaust gas conversion, partial oxidation and reforming of methane will be studied. Morphology of the samples will be analyzed by electron microscopy (SEM, TEM) and X-ray tomography (XRT). Impact of the microstructure on the overall performance of the device will be tested in a lab reactor. The topic is supported by the leading catalyst manufacturer Johnson Matthey.
Faculty of Chemical Engineering

Porous catalytic layers in structured reactors

Kočí Petr, doc. Ing. Ph.D. ( pet...@vscht.cz)
The work focuses on preparation and coating of porous catalytic layers in structured reactors such as honeycomb monoliths, filters, porous membranes and open foams. The aim is to intensify the reactor operation with respect to utilization of the catalytic material and mass and heat tranfer. Industrially relevant processes such as exhaust gas conversion, partial oxidation and reforming of methane will be studied. Morphology of the samples will be analyzed by electron microscopy (SEM, TEM) and X-ray tomography (XRT). Impact of the microstructure on the overall performance of the device will be tested in a lab reactor. The topic is supported by the leading catalyst manufacturer Johnson Matthey.

Synthesis and Production of Drugs - Drugs and Biomaterials

Faculty of Chemical Engineering

Preparation of drug delivery carriers for treatment of rheumatoid arthritis

Šoóš Miroslav, prof. Ing. Ph.D. ( Mir...@vscht.cz)
Skořepová Eliška, Ing. Ph.D. ( sko...@seznam.cz)
Rheumatoid arthritis (RA) is a chronic autoimmune disorder, mainly affecting joints that are identified by inflammation and swelling of the synovium of the joint. Today, apart from the conventional synthetic disease-modifying antirheumatic drugs (DMARDs), a number of biological DMARDs have been approved. Recently the first targeted synthetic DMARD has also been approved, while other targeted compounds are in the development phase. Particularly interesting is group of drugs which are based on gold complexes. Despite their promising properties, these drugs has low solubility in water and thus low bioavailability. Therefore, within this project we plan to investigate possibility to prepare more soluble compounds of gold complexes using crystal engineering approach as well as formulate these drugs into various nanocarriers. Combination of various preparation and analytical techniques will be used to investigate stability of gold complexes. In the next step we will investigate the impact of encapsulation matrix or complexation partner on the dissolution characteristics of gold complexes.

Faculty of Chemical Engineering

Preparation of drug delivery carriers for treatment of rheumatoid arthritis

Šoóš Miroslav, prof. Ing. Ph.D. ( Mir...@vscht.cz)
Rheumatoid arthritis (RA) is a chronic autoimmune disorder, mainly affecting joints that are identified by inflammation and swelling of the synovium of the joint. Today, apart from the conventional synthetic disease-modifying antirheumatic drugs (DMARDs), a number of biological DMARDs have been approved. Recently the first targeted synthetic DMARD has also been approved, while other targeted compounds are in the development phase. Particularly interesting is group of drugs which are based on gold complexes. Despite their promising properties, these drugs has low solubility in water and thus low bioavailability. Therefore, within this project we plan to investigate possibility to prepare more soluble compounds of gold complexes using crystal engineering approach as well as formulate these drugs into various nanocarriers. Combination of various preparation and analytical techniques will be used to investigate stability of gold complexes. In the next step we will investigate the impact of encapsulation matrix or complexation partner on the dissolution characteristics of gold complexes.
Faculty of Chemical Engineering

Preparation of drug delivery carriers for treatment of rheumatoid arthritis

Šoóš Miroslav, prof. Ing. Ph.D. ( Mir...@vscht.cz)
Rheumatoid arthritis (RA) is a chronic autoimmune disorder, mainly affecting joints that are identified by inflammation and swelling of the synovium of the joint. Today, apart from the conventional synthetic disease-modifying antirheumatic drugs (DMARDs), a number of biological DMARDs have been approved. Recently the first targeted synthetic DMARD has also been approved, while other targeted compounds are in the development phase. Particularly interesting is group of drugs which are based on gold complexes. Despite their promising properties, these drugs has low solubility in water and thus low bioavailability. Therefore, within this project we plan to investigate possibility to prepare more soluble compounds of gold complexes using crystal engineering approach as well as formulate these drugs into various nanocarriers. Combination of various preparation and analytical techniques will be used to investigate stability of gold complexes. In the next step we will investigate the impact of encapsulation matrix or complexation partner on the dissolution characteristics of gold complexes.
Faculty of Chemical Engineering

Preparation of porous materials for enzyme immobilization and their application in biocatalysis

Šoóš Miroslav, prof. Ing. Ph.D. ( Mir...@vscht.cz)
Application of enzymes for preparation of various biomolecules is ever growing field. This is due to low energy demand and high specificity of the catalyzed reactions. Significant disadvantage of this technology is loss of enzyme activity and removal of the enzyme from the reaction system. Possible solution for these problems is enzyme immobilization onto a suitable support. In this project we will use recently developed technology of reactive gelation suitable for synthesis of 3D porous material with tunable porosity and pore size distribution combined with covalent attachment of enzymes to the surface of prepare material. To understand impact of enzyme-surface interactions we plan to use various building blocks made out of polymers or silica combined with different spacer molecules placed between porous material and enzyme. In this way we will be able to study impact of these interactions on the enzyme activity and yield of biocatalytic reaction. Once the system will be established, we will further investigate effect of process conditions (dispersed porous aggregates vs. packed bed), effect of ionic strength, pH, substrate concentration etc., on the yield and selectivity of the performed enzymatic reaction. In the last part of the project, the system will be extended towards multiple consequent enzymatically catalyzed reactions. Student will be involved in the preparation of porous material and its characterization as well as in the surface functionalization with suitable enzyme anchoring moieties. Consequently, student will be responsible for enzyme attachment and testing of its activity and yield. Combination of several analytical techniques including SEM, light scattering, BET measurement, Hg porosimetry, XPS, HPLC etc., will be used to characterize material properties and test behavior of immobilized enzymes.
Faculty of Chemical Engineering

Preparation of porous materials for enzyme immobilization and their application in biocatalysis

Šoóš Miroslav, prof. Ing. Ph.D. ( Mir...@vscht.cz)
Application of enzymes for preparation of various biomolecules is ever growing field. This is due to low energy demand and high specificity of the catalyzed reactions. Significant disadvantage of this technology is loss of enzyme activity and removal of the enzyme from the reaction system. Possible solution for these problems is enzyme immobilization onto a suitable support. In this project we will use recently developed technology of reactive gelation suitable for synthesis of 3D porous material with tunable porosity and pore size distribution combined with covalent attachment of enzymes to the surface of prepare material. To understand impact of enzyme-surface interactions we plan to use various building blocks made out of polymers or silica combined with different spacer molecules placed between porous material and enzyme. In this way we will be able to study impact of these interactions on the enzyme activity and yield of biocatalytic reaction. Once the system will be established, we will further investigate effect of process conditions (dispersed porous aggregates vs. packed bed), effect of ionic strength, pH, substrate concentration etc., on the yield and selectivity of the performed enzymatic reaction. In the last part of the project, the system will be extended towards multiple consequent enzymatically catalyzed reactions. Student will be involved in the preparation of porous material and its characterization as well as in the surface functionalization with suitable enzyme anchoring moieties. Consequently, student will be responsible for enzyme attachment and testing of its activity and yield. Combination of several analytical techniques including SEM, light scattering, BET measurement, Hg porosimetry, XPS, HPLC etc., will be used to characterize material properties and test behavior of immobilized enzymes.

Synthesis and Production of Drugs - Drugs and Biomaterials

Faculty of Chemical Engineering

Process scale-up of pharmaceutical spray drying

Štěpánek František, prof. Ing. Ph.D. ( Fra...@vscht.cz)
Spray drying is versatile method for converting solutions, suspensions or pastes into dry, free-flowing powders in the pharmaceutical, food and nutraceutical industries. During product development, the formulation and process variables are typically optimised using a laboratory-scale spray dryer, and the process is then transferred to a pilot or full manufacturing scale. However, it is notoriously difficult to maintain the same particle properties using spray dryers at different scales, which often necessitated long and expensive trials to be carried out at the large scale. The aim of this project is to develop a robust methodology for spray drying process scale-up in an industrial pharmaceutical setting. The main focus will on the transferability of particle size and particle morphology, as these two parameters are known to be the most sensitive to parameters that vary between the laboratory and the manufacturing scale spray-dryers: the initial droplet size and the drying conditions (temperature, gas flowrate, and residence time in the drying chamber).

Faculty of Chemical Engineering

Process scale-up of pharmaceutical spray drying

Štěpánek František, prof. Ing. Ph.D. ( Fra...@vscht.cz)
Spray drying is versatile method for converting solutions, suspensions or pastes into dry, free-flowing powders in the pharmaceutical, food and nutraceutical industries. During product development, the formulation and process variables are typically optimised using a laboratory-scale spray dryer, and the process is then transferred to a pilot or full manufacturing scale. However, it is notoriously difficult to maintain the same particle properties using spray dryers at different scales, which often necessitated long and expensive trials to be carried out at the large scale. The aim of this project is to develop a robust methodology for spray drying process scale-up in an industrial pharmaceutical setting. The main focus will on the transferability of particle size and particle morphology, as these two parameters are known to be the most sensitive to parameters that vary between the laboratory and the manufacturing scale spray-dryers: the initial droplet size and the drying conditions (temperature, gas flowrate, and residence time in the drying chamber).
Faculty of Chemical Engineering

Rate-based model of multi-component distillation. Experimental verification.

Rejl František, doc.Ing. Ph.D. ( r...@vscht.cz)
The current design of rectification columns is based mostly on experience and is essentially empirical in nature. The development of computer technologies has brought some improvements by introducing of the so-called "rate-based" models using numerical integration of the differential momentum, energy and mass balances. The unavailability and unreliability of the corresponding transfer coefficients characterizing the rate of the processes is a major obstacle to their wider use. Current methods of their acquisition – transfer from absorption conditions to distillation ones and using the analogy between heat and mass transfer is risky and requires the use of large safety factors in the design. Also, the Maxwell-Stefan approach to the calculation of the interfacial mass flows from the transfer coefficients obtained for the respective binary systems, which is used to calculate the coefficients in multi-component mixtures, has not yet been experimentally verified. There has been no method for direct determination of the transport coefficients under distillation conditions. At our workplace, a methodology for the direct determination of transport coefficients during distillation has been developed and successfully tested, thus significantly strengthening the possibility of critical evaluation of current procedures for their determination. The aim of the dissertation is to verify the Maxwell-Stefan approach to the calculation of mass transfer coefficients in multi-component mixtures from binary coefficients. The mass transfer coefficients measured in the three binary mixtures forming the ternary mixture, e.g. methanol / ethanol / 1-propanol and the concentration profiles along the packed bed measured for the ternary mixture will be used for verification. The ultimate goal of this research is to refine the design of the columns based on simulation rate-based models using mass transfer transport coefficients measured directly in the rectification column.
Faculty of Chemical Engineering

Solubility and stability of organic redox species for electrochemical energy storage

Kosek Juraj, prof. Dr. Ing. ( Jur...@vscht.cz)
Mazúr Petr, Ing. Ph.D.
Fast development of renewable energy sources and of electromobility requires both stationary distributed energy storage and mobile batteries or fuel cells with high specific energy and sufficient power. PhD student will concentrate on experimental and theoretical research of flow electrochemical systems for the accumulation of electric energy.

The central focus of this project will be the utilization of organic redox species in electrolytes of redox flow batteries. In the cooperation with organic chemists, we are going to search for molecules with suitable properties for their use in stationary and mobile convertors of electric / electrochemical energy with the aim to improve both technical and economic characteristics of energy storages. The emphasis will be given on the understanding of the relation between chemical structure of organic molecules and their relevant properties such as solubility in aqueous and non-aqueous electrolytes, kinetics of electrode reactions and transport of molecules through the separator/membrane. PhD student will also investigate chemical and electrochemical stability of organic redox species at conditions of expected applications, effect of impurities and other aspects. The results of this work shall enable the intensification of energy storage in organic redox flow batteries and the improvement of their economical parameters.

The outcome of this work will be not only series of papers in impacted journals, but also practical knowledge enabling practical utilization of invented redox species in scale-up to pilot-plant units. PhD student will collaborate not only in the team of PhD students and postdocs in our laboratories, but will also cooperate with industrial and academic partners.

Info: phone 220 44 3296, room B-145, e-mail jkk@vscht.cz, webhttp://kosekgroup.cz
Faculty of Chemical Engineering

Study and preparation of nanoparticles in controlled conditions of microfluidic arrangement

Tokárová Viola, Ing. Ph.D. ( vio...@vscht.cz)
Properties of nanoparticles (e.g., optical, electromagnetic or mechanical) depend on particle size, morphology and structure and there is an increasing demand for particles having low polydispersity and thus near-identical properties. Batch-wise particle preparation is one of the most employed techniques due to its straightforward character and feasibility of instrumentation. However, the use of batch-wise synthesis is not ideal for rapid precipitation reaction given by non-ideal mixing of reactants. Mixing efficiency plays a significant role since the nucleation process triggered upon reactant mixing occurs within few milliseconds. Continuous microfluidic reactors are promising technology allowing thanks to their small dimensions, high surface to volume ratio and mixing intensification lowering of the final particle polydispersity and produce particles with superior properties compared to those produced by means of batch-wise preparation. The aim of this work will be to optimize the architecture of microfluidic channels and its effect on the mixing of the reactants with respect to the synthesis of nanoparticles of the required size.
Faculty of Chemical Engineering

Study and preparation of nanoparticles in controlled conditions of microfluidic arrangement

Tokárová Viola, Ing. Ph.D. ( vio...@vscht.cz)
Properties of nanoparticles (e.g., optical, electromagnetic or mechanical) depend on particle size, morphology and structure and there is an increasing demand for particles having low polydispersity and thus near-identical properties. Batch-wise particle preparation is one of the most employed techniques due to its straightforward character and feasibility of instrumentation. However, the use of batch-wise synthesis is not ideal for rapid precipitation reaction given by non-ideal mixing of reactants. Mixing efficiency plays a significant role since the nucleation process triggered upon reactant mixing occurs within few milliseconds. Continuous microfluidic reactors are promising technology allowing thanks to their small dimensions, high surface to volume ratio and mixing intensification lowering of the final particle polydispersity and produce particles with superior properties compared to those produced by means of batch-wise preparation. The aim of this work will be to optimize the architecture of microfluidic channels and its effect on the mixing of the reactants with respect to the synthesis of nanoparticles of the required size.
Faculty of Chemical Engineering

Study of transport characteristics in various types of bioreactors

Moucha Tomáš, prof. Dr. Ing. ( mou...@vscht.cz)
The production of new biotechnology and pharmaceutical products is based on a bioreactor design. The choice of a suitable type of bioreactor is crucial with respect to maximum yield, but it is also limited by the lifetime of the microorganisms present. The aim of the doctoral study is to compare design parameters (transport characteristics) of three types of the most commonly used bioreactors. The results will be used to characterize the differences and similarities of specific types of bioreactors in terms of gas distribution, mass transfer and mixing depending on the total energy supplied to the system. Transport characteristics will be obtained experimentally for model batches, which will be designed based on physical properties of real broths. Both cooperating departments are well equipped and have all the three types of bioreactors i) mechanically stirred reactor, ii) bubbled column and iii) air-lift reactor. All bioreactors are adapted to measure transport characteristics by the same methods, therefore the results will be comparable. Requirements for applicant: master degree in chemical or mechanical engineering, organic technology, biotechnology etc.; ability for teamwork; systematic and creative approach to scientific problems; interest in experimental work Further information: Assoc. Prof. Tomáš Moucha, building B of UCT Prague, room T02, e-mail: tomas.moucha@vscht.cz
Development principles: 1. To study the state of the art in hydrodynamics and mass transfer in stirred tank reactors, bubble columns and air-lift reactors.
2. To acquire the techniques of transport characteristics measurement (parameters describing the liquid mixing in bioreactors, volumetric mass transfer coefficient, gas hold-up, etc.).
3. To provide the systematic measurements of transport characteristics for different model liquids in three types of bioreactors: stirred tank reactor, bubble column and air-lift reactor.
4. According to obtained results, to compare the individual bioreactors, to characterize the similar and different properties and to assess their applicability.
Faculty of Chemical Engineering

Synthesis and characterisation of particles with immuno-adhesive properties

Štěpánek František, prof. Ing. Ph.D. ( Fra...@vscht.cz)
The specificity of adhesion to the target cells or tissues in a physiological envirionment is a key requisite for the successful implementation of drug delivery systems (DDS). The aim of this work is to explore DDS based on immunoliposomes and their composites (e.g. with magnetic nanoparticles) using surface modification by antibody fragments and other suitable targeting moieties. The immunoliposomes will be tested both in vitro and in vivo in terms of specificity of adhesion, pharmacokinetics, and ability do encapsulate and delivery drugs or pro-drugs.

Synthesis and Production of Drugs - Drugs and Biomaterials

Faculty of Chemical Engineering

Synthesis and characterisation of particles with immuno-adhesive properties

Štěpánek František, prof. Ing. Ph.D. ( Fra...@vscht.cz)
Lizoňová Denisa, Ing. ( den...@gmail.com)
The specificity of adhesion to the target cells or tissues in a physiological envirionment is a key requisite for the successful implementation of drug delivery systems (DDS). The aim of this work is to explore DDS based on immunoliposomes and their composites (e.g. with magnetic nanoparticles) using surface modification by antibody fragments and other suitable targeting moieties. The immunoliposomes will be tested both in vitro and in vivo in terms of specificity of adhesion, pharmacokinetics, and ability do encapsulate and delivery drugs or pro-drugs.

Faculty of Chemical Engineering

Synthesis and characterization of nanoparticle system for transfection of cells

Šoóš Miroslav, prof. Ing. Ph.D. ( Mir...@vscht.cz)
Delivery of gene vectors during cell transfection is commonly done by positively charged poly ions. When coupled with DNA, this method is capable to deliver the genetic information into the host cell’s nucleus resulting in the production of the protein of interest. Even though this procedure is commonly used, toxicity of polycations results in low cell viability and loss of the culture. In this project we plan to develop a transfection system based on the biodegradable polymers with low toxicity using recently developed aggregation process. Student will be involved in the selection, synthesis and modification of the biodegradable polymer followed by the preparation of polymeric nanoparticles as a DNA carriers. Properties of the prepared polymer will be characterized via various methods include light scattering or GPC. Formed nanoparticles will be characterized by DLS, SEM/TEM, measurement of zeta potential and their colloidal stability. Consequent complexation of produced NPs with DNA and their size will be tested as well. In the last part of the project, process of complexation will be scaled up to the necessary amount to be tested with living cells.
Faculty of Chemical Engineering

Synthesis and characterization of nanoparticle system for transfection of cells

Šoóš Miroslav, prof. Ing. Ph.D. ( Mir...@vscht.cz)
Delivery of gene vectors during cell transfection is commonly done by positively charged poly ions. When coupled with DNA, this method is capable to deliver the genetic information into the host cell’s nucleus resulting in the production of the protein of interest. Even though this procedure is commonly used, toxicity of polycations results in low cell viability and loss of the culture. In this project we plan to develop a transfection system based on the biodegradable polymers with low toxicity using recently developed aggregation process. Student will be involved in the selection, synthesis and modification of the biodegradable polymer followed by the preparation of polymeric nanoparticles as a DNA carriers. Properties of the prepared polymer will be characterized via various methods include light scattering or GPC. Formed nanoparticles will be characterized by DLS, SEM/TEM, measurement of zeta potential and their colloidal stability. Consequent complexation of produced NPs with DNA and their size will be tested as well. In the last part of the project, process of complexation will be scaled up to the necessary amount to be tested with living cells.

Synthesis and Production of Drugs - Drugs and Biomaterials

Faculty of Chemical Engineering

Synthesis and characterization of nanoparticle system for transfection of cells

Šoóš Miroslav, prof. Ing. Ph.D. ( Mir...@vscht.cz)
Delivery of gene vectors during cell transfection is commonly done by positively charged poly ions. When coupled with DNA, this method is capable to deliver the genetic information into the host cell’s nucleus resulting in the production of the protein of interest. Even though this procedure is commonly used, toxicity of polycations results in low cell viability and loss of the culture. In this project we plan to develop a transfection system based on the biodegradable polymers with low toxicity using recently developed aggregation process. Student will be involved in the selection, synthesis and modification of the biodegradable polymer followed by the preparation of polymeric nanoparticles as a DNA carriers. Properties of the prepared polymer will be characterized via various methods include light scattering or GPC. Formed nanoparticles will be characterized by DLS, SEM/TEM, measurement of zeta potential and their colloidal stability. Consequent complexation of produced NPs with DNA and their size will be tested as well. In the last part of the project, process of complexation will be scaled up to the necessary amount to be tested with living cells.

Faculty of Chemical Engineering

Triboelectric routes enabling plastic waste separation and recycling

Kosek Juraj, prof. Dr. Ing. ( Jur...@vscht.cz)
Zubov Alexandr, Ing. Ph.D. ( Ale...@vscht.cz)
Recycling is the most beneficial and eco-friendly way to treat large amount of plastic waste. However, conversely to a public belief, the majority of plastic waste is burned in incineration plants or stored in landfills instead of being recycled. The bottleneck of the plastic waste treatment originates in the pre-separation, as only precisely separated waste can be recycled. Even the incineration process requires the pre-separation of plastics, mainly the removal of polymers containing halogens that could otherwise form harmful gases during combustion.

Current methods like manual separation, IR spectroscopy or methods based on density differences aren’t sufficiently effective. The new promising technique, triboelectric separation, is based on the idea that each plastic material reaches different electrostatic charge by tribocharging (charging by friction) and therefore charged plastic mixtures can be separated in electric field.

The objective of this Ph.D. project is the establishment of experimental bases (systematic series of data) related to charging and discharging dynamics in powders, which will provide integrated description of these phenomena. The student will also investigate opportunities for control of surface charge and subsequent separation of dielectrics in electric field. The student shall challenge several open problems: (i) relation between ESC and mechanical/chemical properties of materials, (ii) electric charge dissipation, (iii) charging of powders under the conditions simulating real industrial production of industrially important powders, (iv) the effect of charge on fouling, (v) charging for separation and recycling of plastic materials.

The project is a pioneering work which is desperately needed and is sufficiently challenging for a student with interest in physico-chemical fundamentals of previously described processes. The student will work with highly qualified Ph. D. students and postdocs in our research group and will also cooperate with our European partners. Our laboratory is well prepared for the research of electrostatic processes (Faraday cup, corona charging, high-voltage separator) and characterization of powder texture and material properties (micro-tomography, atomic force microscopy – AFM).

Info: phone 220 44 3296, office B-145, e-mailjkk@vscht.cz, web http://kosekgroup.cz
Faculty of Chemical Engineering

CFD simulation of air flow and cross-contamination in production space

Kašpar Ondřej, Ing. Ph.D. ( KAS...@vscht.cz)
Dohnal Jiří, Ing. Ph.D. ( jir...@zentiva.cz)
One of the severe concerns in the modern pharmaceutical industry is the risk of cross-contamination. Cross-contamination refers to a state where foreign substances get into the manufactured product at a certain production process stage. The most dangerous foreign substances are pharmaceutical compounds present in the production premises during the parallel production of another product. This can be prevented by a suitable space layout with good air exchange. CFD computational tools based on the finite element method (FEM) allow the solution of complex physical problems such as the airflow in enclosed spaces and the study of particle deposition dynamics. The work's subject is the use of CFD to calculate velocity fields and study the deposition of model particles representing dust generated during common operations of the pharmaceutical industry, such as tableting or granulation.
Department of Chemical Engineering
Faculty of Chemical Engineering

Formation of Microstructured Materials through Self-Assembly

Řehoř Ivan, RNDr. Ph.D. ( REH...@vscht.cz)
Self-assembly is a spontaneous arrangement of individual units - building blocks - into an ordered structure. The ordered structure has the lowest energy from all accessible building block arrangements, which drives the assembly process. The arrangement of the ordered structure is defined by the properties of the building blocks, such as their shape, material anisotropy or magnetic interaction with external field. Tailoring these properties to achieve desired structure can be considered 'programming' and may represent viable alternative to other ways of constructing micro and nanostructured materials. The question of lengthscales is crucial in self-assembly. When building blocks are small enough (We recently demonstrated, that we can assemble anisotropic hydrogel microparticles on solid liquid interface to form ordered 2D structures. We introduced novel mechanisms to control orientation of the building blocks during the self-assembly process and, thus, to not freeze in the disorderedd state. Ordered microparticles can be subsequently covalently bound together. The resulting structure - sheet - has complex mechanical properties i.e. ability to buckle in a preprogrammed way determined by the shape, size and material composition of the building blocks. The goal of the project is to find new approaches to the self-assembly of hydrogel microparticles and combine them with directed asembly methods using mobile microrobots developed in our team (https://www.youtube.com/watch?v=PQOXS7f9rDg). Resulting structures will find application in the preparation of metamaterials, microrobotics or tissue engineering.
Department of Chemical Engineering
Faculty of Chemical Engineering

Formation of Microstructured Materials through Self-Assembly

Řehoř Ivan, RNDr. Ph.D. ( REH...@vscht.cz)
Self-assembly is a spontaneous arrangement of individual units - building blocks - into an ordered structure. The ordered structure has the lowest energy from all accessible building block arrangements, which drives the assembly process. The arrangement of the ordered structure is defined by the properties of the building blocks, such as their shape, material anisotropy or magnetic interaction with external field. Tailoring these properties to achieve desired structure can be considered 'programming' and may represent viable alternative to other ways of constructing micro and nanostructured materials. The question of lengthscales is crucial in self-assembly. When building blocks are small enough (We recently demonstrated, that we can assemble anisotropic hydrogel microparticles on solid liquid interface to form ordered 2D structures. We introduced novel mechanisms to control orientation of the building blocks during the self-assembly process and, thus, to not freeze in the disorderedd state. Ordered microparticles can be subsequently covalently bound together. The resulting structure - sheet - has complex mechanical properties i.e. ability to buckle in a preprogrammed way determined by the shape, size and material composition of the building blocks. The goal of the project is to find new approaches to the self-assembly of hydrogel microparticles and combine them with directed asembly methods using mobile microrobots developed in our team (https://www.youtube.com/watch?v=PQOXS7f9rDg). Resulting structures will find application in the preparation of metamaterials, microrobotics or tissue engineering.
Department of Chemical Engineering
Faculty of Chemical Engineering

Modular Hydrogel Microrobots

Řehoř Ivan, RNDr. Ph.D. ( REH...@vscht.cz)
Miniturization of robots to the sizes of tens of microns will allow their application in new, currently inacccesible areas, such as controlled drug delivery or microsurgery. In order to simplify the mechatronic design of such microrobots, novel approaches have been adopted for their operation and control, exploiting soft materials, actuating through deformation, such as responsive polymers. These advanced materials, together with development of their processing in microscales gave rise to soft microrobots capable of independent untethered motion or manipulation with other objects . In our group, we have recently developed hydrogel microrobots, crawling over surfaces, powered by light (https://www.youtube.com/watch?v=PQOXS7f9rDg). Many of the visionary real-life applications of microrobots foresee their ability to autonomously cooperate and connect into greater structures, that will perform tasks, inaccessible to the individual robots. Modular connectivity of macroscopic locomotive robots has been tackled experimentaly, demonstrating the extended application of such robot assemblies, compared to the individual robots. The connective mechanisms and the organization of the individual robots into the assembly however remains a complex and challenging task. This project aims to probe potential pathways for the connection of crawling hydrogel microrobots into actuating millimeter-sized structures, capable of performing mechanical work.
Development principles: 1. Zvládnout metodu stop-flow litografie (SFL)
2. Vyrobit pomocí SFL hydrogelové mikroroboty schopné pohybu plazenim po substratu
3. vyvinout metody pro spojování robotů pomocí jejich kovalentní-nekovalentní interakce
4. vyvinout algoritmy pohybu robotů po subrtrátu, které povedou k jejich spojování do větších funkčních celků pomocí metody vyvinuté v bodě 3.
Department of Chemical Engineering
Faculty of Chemical Engineering

Modular Hydrogel Microrobots

Řehoř Ivan, RNDr. Ph.D. ( REH...@vscht.cz)
Miniturization of robots to the sizes of tens of microns will allow their application in new, currently inacccesible areas, such as controlled drug delivery or microsurgery. In order to simplify the mechatronic design of such microrobots, novel approaches have been adopted for their operation and control, exploiting soft materials, actuating through deformation, such as responsive polymers. These advanced materials, together with development of their processing in microscales gave rise to soft microrobots capable of independent untethered motion or manipulation with other objects . In our group, we have recently developed hydrogel microrobots, crawling over surfaces, powered by light (https://www.youtube.com/watch?v=PQOXS7f9rDg). Many of the visionary real-life applications of microrobots foresee their ability to autonomously cooperate and connect into greater structures, that will perform tasks, inaccessible to the individual robots. Modular connectivity of macroscopic locomotive robots has been tackled experimentaly, demonstrating the extended application of such robot assemblies, compared to the individual robots. The connective mechanisms and the organization of the individual robots into the assembly however remains a complex and challenging task. This project aims to probe potential pathways for the connection of crawling hydrogel microrobots into actuating millimeter-sized structures, capable of performing mechanical work.
Development principles: 1. Zvládnout metodu stop-flow litografie (SFL)
2. Vyrobit pomocí SFL hydrogelové mikroroboty schopné pohybu plazenim po substratu
3. vyvinout metody pro spojování robotů pomocí jejich kovalentní-nekovalentní interakce
4. vyvinout algoritmy pohybu robotů po subrtrátu, které povedou k jejich spojování do větších funkčních celků pomocí metody vyvinuté v bodě 3.
Department of Chemical Engineering
Updated: 11.12.2019 11:36, Author: Jan Kříž

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