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Data for 2018/2019

Molecular genetics and DNA analysis

Credits 3
Hours per week 2 / 0 / 0
Examination Ex
Study Language English
Level Bachelor subject
Guarantor prof. Ing. Tomáš Ruml, CSc.
Supplemental electronic materials available at UCT Prague's e-learning

Summary

The subject is focused on understanding heredity principles especially mechanisms and regulation of transfer of genetic information and gene expression in prokaryotic and eukaryotic organisms. Special emphasis will be put on the main regulatory processes mediated by proteins and RNA regulatory molecules. Other focus is on understanding the variability of genetic information due to mutations and recombination. The subject offers information on the most important methods of DNA analysis as DNA and RNA isolation, their labeling, sequencing and analysis of selected sequences by using polymerase chain reaction and comparison of data with available databases.

Syllabus

1. Historic overview and basic genetic laws and terms.
2. Structure and function of nucleic acids, genome organization
3. Replication, DNA polymerases, replisome complex.
4. Mutations and repair mechanisms.
5. Recombination, mitotic and meiotic gene segregation, transposition.
6. Regulation of transcription.
7. Posttranscriptional modifications of RNA.
8. Regulatory role of RNA.
9. Translation, posttranslational modifications.
10. Principles of work with DNA and RNA.
11. Methods of analysis of DNA sequence, genome mapping.
12. Labeling of nucleic acids, application of probes.
13. Polymerase chain reaction and its applications.
14. DNA databases.

Literature

R: Lodish, H.; et al. Molecular cell biology, 6th ed.;W.H.Freeman & Co Ltd, 2008.
R: Albrets B; et al. Molecular biology of the cell, 5th ed. Garland Science, 2008.
R: Clark D.P. Molecular biology, Elsevier Inc., 2005.
R: Hitler J.M. Forensic DNA Typing, 2nd ed. Elsevier 2005.

UCT Prague
Technická 5
166 28 Prague 6 – Dejvice
IČO: 60461373
VAT: CZ60461373

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