Department of Biochemistry and Microbiology
List of available PhD theses
Production of antibacterial peptides from marine organisms and evaluation of their biological activity
Design of bacterial expression system for production of existing antibacterial peptides identified in the laboratory of prof. Stensvåg at University in Tromso. Firstly, it is necessary to overcome the toxicity issues either by fusion with bulky protein or secretion achieved by fusion of the peptides to export sequence. Next step is upscale to massive production and purification for functional studies (pull down assay with bacterial cell lysate). Isolated products will be used for analysis of biological activity targeted against bacterial and yeast cells, including biofilms and determination of immunomodulatory effects. Subsequently it is planned a study of mechanisms leading to inhibition.